Anti-Topoisomerase II alpha/TOP2A Antibody
Rabbit IgG polyclonal antibody for DNA topoisomerase 2-alpha(TOP2A) detection. Tested with WB in Human.
Anti-Topoisomerase II alpha/TOP2A Antibody Info At A Glance
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
|Product Name||Anti-Topoisomerase II alpha/TOP2A Antibody
See all TOP2A primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for TOPOISOMERASE II ALPHA/TOP2A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. TOPOISOMERASE II ALPHA/TOP2A information: Molecular Weight: 174385 MW; Subcellular Localization: Cytoplasm . Nucleus, nucleoplasm . Generally located in the nucleoplasm.|
|Cite This Product||Anti-Topoisomerase II alpha/TOP2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1127)|
|Similar Products From
|Anti-Topoisomerase II alpha/TOP2A Antibody may replace the following items: sc 87488|sc 87487.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha(1466-1480aa RKPSTSDDSDSNFEK), different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For TOP2A (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7153|
|Species Of This Entry||Human|
|Protein Name||DNA topoisomerase 2-alpha|
|Superfamily||type II topoisomerase family|
|Alternative Names||TOP2A|DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 18.104.22.168; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A|
|Post Tranlational Modifications||Phosphorylation; Methylation; Acetylation; Cleavage; Oxidation; Dephosphorylation; Ubiquitination|
|Subcellular Localization||Cytoplasm. Nucleus, nucleoplasm. Generally located in the nucleoplasm.|
|Gene Location||17q21.2, on Chromasome 17, gene sequence: NC_000017.11 (40388525..40417902, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TOP2A (Source: Uniprot.org, NCBI)
|Protein Function||Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).|
|Interacting Proteins||BRCA1; CTNNB1; PRKCD|
|Research Areas||Cancer; Cell Cycle and Replication; Core ESC-Like Genes; DNA Repair; Phospho-Specific; Stem Cell Markers|
|Related Diseases||Malignant Neoplasms; Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Carcinoma; Neoplasm Metastasis; Adenocarcinoma; Malignant Paraganglionic Neoplasm; Leukemia; Cell Transformation, Neoplastic; Malignant Neoplasm Of Ovary; Ovarian Neoplasm; Lymphatic Metastasis; Ductal Breast Carcinoma; Lung Neoplasms; Malignant Neoplasm Of Lung; Invasive Breast Carcinoma; Liver Carcinoma; Ductal Carcinoma|
|Related Pathways||Cell Proliferation; Cell Cycle; Drug Resistance; Pathogenesis; Dna Replication; Dna Repair; Localization; Translation; Cell Division; Chromosome Condensation; Reverse Transcription; Mitosis; Metaphase; Methylation; Cell Adhesion; Interphase; Immune Response; Chromosome Segregation; Cell Death; Cell Growth|
|Background||The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.|
|Scientific References||PMID: 2845399 by Tsai-Pflugfelder M., et al. Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.|
PMID: 8393377 by Wasserman R.A., et al. Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast.
Our Boster Quality Guarantee for Anti-Topoisomerase II alpha/TOP2A Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Topoisomerase II alpha (PA1127) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 174KD
Observed bind size: 174KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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