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- Table of Contents
DNA topoisomerase 2-alpha Antibodies
AND TOP2A ELISA kits, DNA topoisomerase 2-alpha Proteins
Many biological assays use antibodies to detect DNA topoisomerase 2-alpha, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DNA topoisomerase 2-alpha in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DNA topoisomerase 2-alpha antibodies...
Designing an IHC experiment for DNA topoisomerase 2-alpha? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the TOP2A IHC GuideTOP2A Infographic by Boster Bio
All TOP2A Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00953
100 μg/vial
Cat # A00953
100 μg/vial
Human, Mouse, Rat
IP, IHC, WB
$370
Cat # M00953-1
100 μl
Cat # M00953-1
100 μl
Human, Mouse, Rat
ELISA
$540
Cat # EKC2629
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2629
1 kit, containing two 96-well plates and all necessary reagents
Human
ELISA
$540
Cat # EKC2591
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2591
1 kit, containing two 96-well plates and all necessary reagents
Human
ELISA
$540
Cat # EKC2312
1 kit, containing two 96-well plates and all necessary reagents
Cat # EKC2312
1 kit, containing two 96-well plates and all necessary reagents
Human
ELISA
$480
Cat # EKC1571
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1571
1 kit, containing one 96-well plate and all necessary reagents
Human
$960
Cat # LS007153
20 µg, 100 µg
Cat # LS007153
20 µg, 100 µg
TOP2A Overview
Facts about DNA topoisomerase 2-alpha.
DNA topoisomerase 2-alpha (TOP2A)
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Gene Information
| Human | |
|---|---|
| Gene Name: | TOP2A |
| Uniprot: | P11388 |
| Entrez: | 7153 |
Family
| Belongs to: |
|---|
| type II topoisomerase family |
Alternative Names
DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A
Molecular Weight
Mass (kDA):
174.385 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 17q21.2 |
| Sequence: | 17; NC_000017.11 (40388525..40417902, complement) |
Subcellular Localizations
Cytoplasm. Nucleus, nucleoplasm. Generally located in the nucleoplasm.
Diseases
- Malignant Neoplasms
- Neoplasms
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Carcinoma
- Neoplasm Metastasis
- Adenocarcinoma
- Malignant Paraganglionic Neoplasm
- Leukemia
- Cell Transformation, Neoplastic
- Malignant Neoplasm Of Ovary
- Ovarian Neoplasm
Interacting Proteins
- BRCA1
- CTNNB1
- PRKCD
Pathways
- Cell Proliferation
- Cell Cycle
- Drug Resistance
- Pathogenesis
- Dna Replication
- Dna Repair
- Localization
- Translation
- Cell Division
- Chromosome Condensation
- Reverse Transcription
- Mitosis
- Metaphase
PTMs
- Phosphorylation
- Methylation
- Acetylation
Research Areas
- Cancer
- Cell Cycle and Replication
- Core ESC-Like Genes
- DNA Repair
- Phospho-Specific
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TOP2A
bosterbio.com/bosterbio-gene-info-cards/TOP2A
References
PMID: 2845399 by Tsai-Pflugfelder M., et al. Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.
PMID: 8393377 by Wasserman R.A., et al. Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast.