Anti-Bag5 Antibody Picoband™

BAG5 antibody

Boster Bio Anti-Bag5 Antibody Picoband™ catalog # A07402. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07402
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-Bag5 Antibody Picoband™

View all BAG5 Antibodies

SKU/Catalog Number

A07402

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bag5 Antibody Picoband™ catalog # A07402. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bag5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07402)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Bag5 recombinant protein (Position: N389-Y447). Human Bag5 shares 96.6% and 93.2% amino acid (aa) sequence identity with mouse and rat Bag5, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07402 is reactive to BAG5 in Human, Mouse, Rat

Applications

A07402 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

51.2kDa

Background of BAG5

BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. It is mapped to 14q32.33. The protein encoded by this gene is a member of the BAG1-related protein family. Bag5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BAG5 (Source: Uniprot.org, NCBI)

Gene Name

BAG5

Full Name

BAG family molecular chaperone regulator 5

Weight

51.2kDa

Alternative Names

BAG family molecular chaperone regulator 5; BAG-5; BAG-family molecular chaperone regulator-5; Bcl-2-associated athanogene 5; BCL2-associated athanogene 5; KIAA0873 BAG5 BAG-5 BAG cochaperone 5 BAG family molecular chaperone regulator 5|BCL2 associated athanogene 5|bcl-2-associated athanogene 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BAG5, check out the BAG5 Infographic

BAG5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BAG5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Bag5 Antibody Picoband™

Question

Does anti-Bag5 antibody A07402 work on monkey WB with testis?

B. Jones

Verified customer

Asked: 2017-02-16

Answer

Our lab technicians have not tested anti-Bag5 antibody A07402 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Bag5 antibody A07402 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey testis in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-02-16

Question

I have a question about product A07402, anti-Bag5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Carter

Verified customer

Asked: 2016-10-13

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07402 anti-Bag5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-10-13

Question

Is there a BSA free version of anti-Bag5 antibody A07402 available?

A. Zhao

Verified customer

Asked: 2013-04-01

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bag5 antibody A07402 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-04-01

Question

Here is the WB image, lot number and protocol we used for testis using anti-Bag5 antibody A07402. Please let me know if you require anything else.

M. Jha

Verified customer

Asked: 2013-01-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-01-03

Order DetailsPrice
A07402

100μg

$370
A07402-10ug

10μg sample (liquid)

$99
A07402-Biotin

100 μg Biotin conjugated

$570
A07402-Cy3

100 μg Cy3 conjugated

$570
A07402-Dylight488

100 μg Dylight488 conjugated

$570
A07402-Dylight550

100 μg Dylight550 conjugated

$570
A07402-Dylight594

100 μg Dylight594 conjugated

$570
A07402-FITC

100 μg FITC conjugated

$570
A07402-HRP

100 μg HRP conjugated

$570
A07402-APC

100 μg APC conjugated

$670
A07402-PE

100 μg PE conjugated

$670
A07402-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A07402
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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