Product Info Summary
SKU: | PA2101 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Bag5 Antibody
SKU/Catalog Number
PA2101
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bag5 Antibody catalog # PA2101. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bag5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2101)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BAG5, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2101 is reactive to BAG5 in Human, Mouse, Rat
Applications
PA2101 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
51 kDa
Calculated molecular weight
51.2kDa
Background of BAG5
BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. The protein encoded by this gene is a member of the BAG1-related protein family. Bag5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BAG5 using anti-BAG5 antibody (PA2101).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAG5 antigen affinity purified polyclonal antibody (Catalog # PA2101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAG5 at approximately 51 kDa. The expected band size for BAG5 is at 51 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of A431 cells using anti-BAG5 antibody (PA2101).
Overlay histogram showing A431 cells stained with PA2101 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAG5 Antibody (PA2101, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BAG5 (Source: Uniprot.org, NCBI)
Gene Name
BAG5
Full Name
BAG family molecular chaperone regulator 5
Weight
51.2kDa
Alternative Names
BAG family molecular chaperone regulator 5; BAG-5; BAG-family molecular chaperone regulator-5; Bcl-2-associated athanogene 5; BCL2-associated athanogene 5; KIAA0873 BAG5 BAG-5 BAG cochaperone 5 BAG family molecular chaperone regulator 5|BCL2 associated athanogene 5|bcl-2-associated athanogene 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAG5, check out the BAG5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAG5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bag5 Antibody (PA2101)
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No publications found for PA2101
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Bag5 Antibody
Question
We are currently using anti-Bag5 antibody PA2101 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The anti-Bag5 antibody (PA2101) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19