Anti-Topoisomerase II alpha/TOP2A Antibody Picoband™

TOP2A antibody

Boster Bio Anti-Topoisomerase II alpha/TOP2A Antibody Picoband™ catalog # A00953. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A00953
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Topoisomerase II alpha/TOP2A Antibody Picoband™

View all TOP2A Antibodies

SKU/Catalog Number

A00953

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Topoisomerase II alpha/TOP2A Antibody Picoband™ catalog # A00953. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Topoisomerase II alpha/TOP2A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00953)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Topoisomerase II alpha/TOP2A, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00953 is reactive to TOP2A in Human

Applications

A00953 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

174 kDa

Calculated molecular weight

174.385kDa

Background of TOP2A

The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract1. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For TOP2A (Source: Uniprot.org, NCBI)

Gene Name

TOP2A

Full Name

DNA topoisomerase 2-alpha

Weight

174.385kDa

Superfamily

type II topoisomerase family

Alternative Names

DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; TOP2; TOP2A; TOP2DNA gyrase; topoisomerase (DNA) II alpha (170kD); topoisomerase (DNA) II alpha 170kDa; TP2A TOP2A TOP2, TP2A DNA topoisomerase II alpha DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|DNA topoisomerase II, 170 kD|DNA topoisomerase II, alpha isozyme|topoisomerase (DNA) II alpha 170kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TOP2A, check out the TOP2A Infographic

TOP2A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TOP2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Topoisomerase II alpha/TOP2A Antibody Picoband™

Question

We ordered your anti-Topoisomerase II alpha/TOP2A antibody for Flow Cytometry on leukemic t-cell a few years ago. I am using human, and I plan to use the antibody for WB next. My lab would like examining leukemic t-cell as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

I looked at the website and datasheets of our anti-Topoisomerase II alpha/TOP2A antibody and I see that A00953 has been tested on human in both Flow Cytometry and WB. Thus A00953 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-18

Question

My boss were well pleased with the WB result of your anti-Topoisomerase II alpha/TOP2A antibody. However we have observed positive staining in ovarian carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is TOP2A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

From literature, ovarian carcinoma does express TOP2A. Generally TOP2A expresses in cytoplasm. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2019-06-24

Question

We are currently using anti-Topoisomerase II alpha/TOP2A antibody A00953 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-29

Answer

The anti-Topoisomerase II alpha/TOP2A antibody (A00953) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-29

Question

I am looking for using your anti-Topoisomerase II alpha/TOP2A antibody for cellular response to dna damage stimulus studies. Has this antibody been tested with western blotting on a549 whole cell lysates? We would like to see some validation images before ordering.

B. Johnson

Verified customer

Asked: 2017-04-06

Answer

Thank you for your inquiry. This A00953 anti-Topoisomerase II alpha/TOP2A antibody is validated on human a549, a549 whole cell lysates, u2os whole cell lysates, k562 whole cell lysates, 293t cells. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-04-06

Question

We have observed staining in human cervix carcinoma erythroleukemia. Any tips? Is anti-Topoisomerase II alpha/TOP2A antibody supposed to stain cervix carcinoma erythroleukemia positively?

J. Carter

Verified customer

Asked: 2014-01-27

Answer

Based on literature cervix carcinoma erythroleukemia does express TOP2A. Based on Uniprot.org, TOP2A is expressed in heart, ovarian carcinoma, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have TOP2A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 10942766, 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332

Boster Scientific Support

Answered: 2014-01-27

Order DetailsPrice
A00953

100μg

$370
A00953-10ug

10μg sample (liquid)

$99
A00953-Biotin

100 μg Biotin conjugated

$570
A00953-Cy3

100 μg Cy3 conjugated

$570
A00953-Dylight488

100 μg Dylight488 conjugated

$570
A00953-Dylight550

100 μg Dylight550 conjugated

$570
A00953-Dylight594

100 μg Dylight594 conjugated

$570
A00953-FITC

100 μg FITC conjugated

$570
A00953-HRP

100 μg HRP conjugated

$570
A00953-APC

100 μg APC conjugated

$670
A00953-PE

100 μg PE conjugated

$670
A00953-carrier-free

Carrier Free

$370

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