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- Table of Contents
Traf6 antibody (A00185) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 11 Publications
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Flow Cytometry analysis of HEPA 1-6 cells using anti-TRAF6 antibody (A00185).
Overlay histogram showing HEPA 1-6 cells stained with A00185 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF6 Antibody (A00185, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA 1-6 cells stained with A00185 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF6 Antibody (A00185, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TRAF6 Antibody Picoband®
Traf6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Traf6 antibody was positively detected by WB in:
rat RH35 whole cell, mouse thymus tissue, mouse spleen tissue, mouse RAW2467 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Traf6 antibody was positively detected by IHC in:
rat intestine tissue, mouse intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Traf6 antibody was positively detected by FC in:
HEPA 1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Mouse
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TRAF6 Antibody Picoband®
View all TRAF6 Antibodies
View all TRAF6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TRAF6 recombinant protein (Position: Q14-D512).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60-65 kDa
Calculated Molecular Weight
60.1 kDa
Background of Traf6
TNF receptor-associated factor 6, also called E3 ubiquitin-protein ligase TRAF6 or RNF85, is a TRAF human protein. The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This gene is mapped to 11p12. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein.
Cite This Product
Anti-TRAF6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00185)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
96%
High Homology
Rabbit
90%
High Homology
Human
88%
Moderate Homology
Cow
88%
Moderate Homology
Dog
88%
Moderate Homology
Monkey
87%
Moderate Homology
Sheep
87%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Traf6 antibody
Immunohistochemistry (IHC) protocol for Traf6 antibody
Flow Cytometry (FC) protocol for Traf6 antibody
Publications
Application:
Species:
AACRNL evolved from virulence factor to epigenetic parasite driving genome expansion in free-living eukaryotes
Published: 2026-01-29
Journal: Nature Communications
Application: WB
Impact Factor: 15.70
Species: Human,Miichthys miiuy
PMID: 41605925
PIF/harbinger transposon-derived protein promotes 7SL expression to enhance pathogen resistance
Published: 2025-01-30
Journal: EMBO REPORTS
Application: WB
Impact Factor: 6.20
Species: Fish
PMID: 39885293
Sulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12
Published: 2024-03-26
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY
Application: WB
Impact Factor: 3.30
Species: Human
PMID: 38537874
The protective effect of rutin against lipopolysaccharide induced acute lung injury in mice based on the pharmacokinetic and pharmacodynamic combination model
Published: 2021-11-24
Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Application: WB
Impact Factor: 3.94
Species: Mouse
PMID: 34839052
microRNA-20-1 and miR-101a suppress the NF-κB-mediated inflammation production by targeting TRAF6 in miiuy croaker | Infection and Immunity
Published: 2021-11-08
Journal: INFECTION AND IMMUNITY
Application: WB
Impact Factor: 3.44
Species: Miiuy croaker
PMID: 34748368
Zi Qi Decoction Alleviates Liver Fibrosis by Inhibiting the Toll-Like Receptor 4 (TLR4)-Related Nuclear Factor kappa b (NF-κB) and Mitogen-Activated Protein Kinase (MAPK) Signaling Pathways
Published: 2021-04-14
Journal: MEDICAL SCIENCE MONITOR
Application: WB
Impact Factor: 2.65
Species: Rat
PMID: 33850093
LncRNA NEAT1 promotes apoptosis and inflammation in LPS‑induced sepsis models by targeting miR‑590‑3p
Published: 2020-07-29
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.79
Species: Human
PMID: 32855700
Anti-inflammation effects of injectable platelet-rich fibrin via macrophages and dendritic cells
Published: 2019-08-26
Journal: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Application: WB
Impact Factor: 3.22
Species: Mouse
PMID: 31449340
Rubiadin-1-methyl ether from Morinda officinalis How. Inhibits osteoclastogenesis through blocking RANKL-induced NF-κB pathway
Published: 2018-11-02
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.56
Species: Mouse
PMID: 30392907
Xuebijing exerts protective effects on lung permeability leakage and lung injury by upregulating Toll-interacting protein expression in rats with sepsis
Published: 2014-09-23
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 1.88
Species: Mouse,Rat
PMID: 25269519
Ligusticum wallichii Extract Inhibited the Expression of IL-1β after AMI in Rats
Published: 2014-08-17
Journal: Evidence-based Complementary and Alternative Medicine
Application: IHC
Impact Factor: 2.18
Species: Rat
PMID: 25202335
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Product Promise
We promise Anti-TRAF6 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00185
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026