Anti-Von Willebrand Factor/VWF Antibody Picoband®

Anti-VWF Picoband antibody, PB9273, Western blotting
All lanes: Anti VWF (PB9273) at 0.5ug/ml
WB: Recombinant Mouse VWF Protein 0.5ng
Predicted bind size: 37KD
Observed bind size: 37KD

Anti-VWF Picoband antibody, PB9273, Western blotting
All lanes: Anti VWF (PB9273) at 0.5ug/ml
WB: Mouse Lung Tissue Lysate at 50ug
Predicted bind size: 309KD
Observed bind size: 309KD

Anti-VWF Picoband antibody, PB9273, IHC(P)
IHC(P): Mouse Liver Tissue

Representative images showed IHC staining of HIF-1α, VEGF, and vWF in the cerebral cortex (magnification 400 ×)
Index in PubMed under a CC BY license. PMID: 38600597

The detection of ovarian angiogenesis in different treatment groups at day 28 after transplantation. a Histochemical images displayed vWF- and CD34-positive cells in ovarian stroma from different treatment groups. Scale bar 50 μm and 25 μm. b MVD quantification determined by CD34- and vWF-positive cells showed the number of microvessels in ovarian sections of the different groups. * p < 0.05 vs. Bu/Cy group; ## p < 0.01, #### p < 0.0001 vs. SA-BG group; $$$ p < 0.0005, $$$$ p < 0.0001 vs. SA-BG/hAECs group
Index in PubMed under a CC BY license. PMID: 33794993

Effects of hAECs on angiogenesis in the injured uteruses. a IHC staining of von Willebrand factor (vWF) reflected the blood vessel density in the uterine scars of different groups at day 30 and 60 after injection of hAECs or PBS ( n = 8 uterine horns per group). Scale bars = 25 μm. b IHC staining of VEGFA in the uterine scars at day 30 and 60 after injection of hAECs or PBS in different groups ( n = 8 uterine horns per group). Scale bars = 25 μm. c Statistical analysis of the blood vessel density indicated by vWF-positive staining. d The VEGFA expression level was semi-qualified by calculating the percentage of positive cells per field under a magnification of × 400. e Western blot analysis showed that VEGFA expression of the uterine scars in the hAECs group and PBS group at day 5 after injection of hAECs or PBS. f The grayscale values of the western blots were analyzed. The protein level of VEGFA was normalized to that of β-tubulin ( n = 5) (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS, P ≥ 0.05)
Index in PubMed under a CC BY license. PMID: 33762002

hAECs facilitated endometrial recovery in the IUA model. A IHC staining of vWF reflected the MVD of the endometrium. The microvessels, which were vWF-positive, are indicated by arrows in the figure. MVD was reduced in the IUA group and increased in the hAEC-treated group. B IHC staining showed that the expression of VEGF was higher in the hAEC-treated group than in the IUA group. C The expression of PCNA decreased in the IUA group and reached almost normal levels in the hAEC-treated group. a–c, scale bar = 100 μm; d–f, scale bar = 50 μm
Index in PubMed under a CC BY license. PMID: 31412924

hAECs facilitated endometrial recovery in the IUA model. A According to IHC staining, the number of ER-positive cells was higher in the hAEC-treated group than in the IUA group. B There was no difference in PR expression among these three groups. C VEGF expression was semi-quantified, and the number of positive cells per field was calculated. D MVD was valued by counting microvascular vessels, which were vWF-positive. E – G . PCNA, ER, and PR expression levels were semi-quantified by calculating the percentage of positive cells per field (* p < 0.05; ** p < 0.01; *** p < 0.001; NS, p ≥ 0.05). a–c, scale bar = 100 μm; d–f, scale bar = 50 μm
Index in PubMed under a CC BY license. PMID: 31412924

IHC analysis of VWF using anti-VWF antibody (PB9273).
VWF was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9273) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

IF analysis of VWF using anti-VWF antibody (PB9273).
VWF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VWF Antibody (PB9273) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of VWF and alpha-Smooth Muscle Actin using anti-VWF antibody (PB9273) and anti-alpha-Smooth Muscle Actin antibody (MA1106).
VWF and alpha-Smooth Muscle Actin a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VWF antibody (PB9273) and mouse anti-alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.