Anti-IL18 binding protein/Il18bp Antibody Picoband™

IL18BP antibody

Boster Bio Anti-IL18 binding protein/Il18bp Antibody Picoband™ catalog # A07261-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A07261-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-IL18 binding protein/Il18bp Antibody Picoband™

View all IL18BP Antibodies

SKU/Catalog Number

A07261-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL18 binding protein/Il18bp Antibody Picoband™ catalog # A07261-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL18 binding protein/Il18bp Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07261-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse IL18 binding protein recombinant protein (Position: T29-A193).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07261-1 is reactive to IL18BP in Mouse, Rat

Applications

A07261-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

21.099kDa

Background of IL18BP

Interleukin-18-binding protein is a protein that in humans is encoded by the IL18BP gene. The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For IL18BP (Source: Uniprot.org, NCBI)

Gene Name

IL18BP

Full Name

Interleukin-18-binding protein

Weight

21.099kDa

Alternative Names

IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa IL18BP FVHa, IL18BP interleukin 18 binding protein interleukin-18-binding protein|MC51L-53L-54L homolog gene product|tadekinig-alfa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL18BP, check out the IL18BP Infographic

IL18BP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL18BP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-IL18 binding protein/Il18bp Antibody Picoband™

Question

Is there a BSA free version of anti-IL18 binding protein/Il18bp antibody A07261-1 available?

Verified Customer

Verified customer

Asked: 2020-03-24

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL18 binding protein/Il18bp antibody A07261-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-24

Question

My question regarding product A07261-1, anti-IL18 binding protein/Il18bp antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07261-1 anti-IL18 binding protein/Il18bp antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-27

Question

We are currently using anti-IL18 binding protein/Il18bp antibody A07261-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

The anti-IL18 binding protein/Il18bp antibody (A07261-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-19

Question

Is this A07261-1 anti-IL18 binding protein/Il18bp antibody reactive to the isotypes of IL18BP?

J. Evans

Verified customer

Asked: 2020-02-05

Answer

The immunogen of A07261-1 anti-IL18 binding protein/Il18bp antibody is E. coli-derived mouse IL18 binding protein recombinant protein (Position: T29-A193). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-05

Question

Would anti-IL18 binding protein/Il18bp antibody A07261-1 work on goat WB with t-cell?

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

Our lab technicians have not validated anti-IL18 binding protein/Il18bp antibody A07261-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IL18 binding protein/Il18bp antibody A07261-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-18

Question

Would A07261-1 anti-IL18 binding protein/Il18bp antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-11-28

Answer

You can see on the product datasheet, A07261-1 anti-IL18 binding protein/Il18bp antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-11-28

Question

Will anti-IL18 binding protein/Il18bp antibody A07261-1 work for WB with leukocyte?

L. Rodriguez

Verified customer

Asked: 2013-01-25

Answer

According to the expression profile of leukocyte, IL18BP is highly expressed in leukocyte. So, it is likely that anti-IL18 binding protein/Il18bp antibody A07261-1 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2013-01-25

Order DetailsPrice
A07261-1

100μg

$370
A07261-1-10ug

10μg sample (liquid)

$99
A07261-1-Biotin

100 μg Biotin conjugated

$570
A07261-1-Cy3

100 μg Cy3 conjugated

$570
A07261-1-Dylight488

100 μg Dylight488 conjugated

$570
A07261-1-Dylight550

100 μg Dylight550 conjugated

$570
A07261-1-Dylight594

100 μg Dylight594 conjugated

$570
A07261-1-FITC

100 μg FITC conjugated

$570
A07261-1-HRP

100 μg HRP conjugated

$570
A07261-1-APC

100 μg APC conjugated

$670
A07261-1-PE

100 μg PE conjugated

$670
A07261-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A07261-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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