Product Info Summary
SKU: | LS010239 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of adaptor-related protein complex 3, sigma 2 subunit (AP3S2) |
Product info
Product Name
AP3S2 (NM_005829) Human Over-expression Lysate
SKU/Catalog Number
LS010239
Size
100 µg
Description
Transient overexpression lysate of adaptor-related protein complex 3, sigma 2 subunit (AP3S2)
Storage & Handling
Cite This Product
AP3S2 (NM_005829) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010239)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
AP3S2 Human Over-expression Lysates NM_005829.
Western blot validation of overexpression lysate (LS010239) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AP3S2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AP3S2 (Source: Uniprot.org, NCBI)
Gene Name
AP3S2
Full Name
AP-3 complex subunit sigma-2
Weight
22.017kDa
Superfamily
adaptor complexes small subunit family
Alternative Names
Adapter-related protein complex 3 sigma-2 subunit; adaptor complex sigma3B; adaptor-related protein complex 3, sigma 2 subunit; AP-3 complex subunit sigma-2; AP-3 complex subunit sigma-3B; AP3S3; clathrin-associated/assembly/adaptor protein, small 4, 22-kD; FLJ35955; sigma3b; sigma-3B-adaptin; Sigma3B-adaptin; Sigma-adaptin 3b AP3S2 AP3S3, sigma3b adaptor related protein complex 3 subunit sigma 2 AP-3 complex subunit sigma-2|AP-3 complex subunit sigma-3B|adapter-related protein complex 3 subunit sigma-2|adaptor complex sigma3B|adaptor related protein complex 3 sigma 2 subunit|clathrin-associated/assembly/adaptor protein, small 4, 22-kD|sigma-3B-adaptin|sigma-adaptin 3b
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AP3S2, check out the AP3S2 Infographic
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In this infographic, you will see the following information for AP3S2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AP3S2 (NM_005829) Human Over-expression Lysate (LS010239)
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No publications found for LS010239
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