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Product Info Summary
| SKU: | LS060489 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G) |
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Product info
Product Name
APOBEC3G (NM_021822) Human Over-expression Lysate
SKU/Catalog Number
LS060489
Size
100 µg
Description
Transient overexpression lysate of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G)
Storage & Handling
Cite This Product
APOBEC3G (NM_021822) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS060489)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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APOBEC3G Human Over-expression Lysates NM_021822.
Western blot validation of overexpression lysate (LS060489) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with APOBEC3G (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for APOBEC3G (Source: Uniprot.org, NCBI)
Gene Name
APOBEC3G
Full Name
DNA dC->dU-editing enzyme APOBEC-3G
Weight
46.408kDa
Superfamily
cytidine and deoxycytidylate deaminase family
Alternative Names
APOBEC-related cytidine deaminase; APOBEC-related protein 9; APOBEC-related protein; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; ARCD; ARP-9; bK150C2.7; CEM-15; CEM15ARP9; dJ494G10.1; DNA dC->dU-editing enzyme APOBEC-3G; EC 3.5.4; EC 3.5.4.-; EC 3.5.4.5; FLJ12740DNA dC->dU editing enzyme; MDS019; phorbolin-like protein MDS019 APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1 apolipoprotein B mRNA editing enzyme catalytic subunit 3G DNA dC->dU-editing enzyme APOBEC-3G|APOBEC-related cytidine deaminase|APOBEC-related protein 9|DNA dC->dU editing enzyme|apolipoprotein B editing enzyme catalytic polypeptide-like 3G|apolipoprotein B mRNA editing enzyme cytidine deaminase|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G|apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G|deoxycytidine deaminase|phorbolin-like protein MDS019
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on APOBEC3G, check out the APOBEC3G Infographic
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In this infographic, you will see the following information for APOBEC3G: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For APOBEC3G (NM_021822) Human Over-expression Lysate (LS060489)
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