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Product Info Summary
| SKU: | LS050807 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) |
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Product info
Product Name
ASAP1 (NM_018482) Human Over-expression Lysate
SKU/Catalog Number
LS050807
Size
100 µg
Description
Transient overexpression lysate of ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1)
Storage & Handling
Cite This Product
ASAP1 (NM_018482) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS050807)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ASAP1 Human Over-expression Lysates NM_018482.
Western blot validation of overexpression lysate (LS050807) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ASAP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Asap1 (Source: Uniprot.org, NCBI)
Gene Name
Asap1
Full Name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Weight
127.421kDa
Alternative Names
130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activatingprotein; AMAP1; ARF GTPase-activating protein 1; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; ArfGAP with SH3 domain, ankyrin repeat and PH domain 1; centaurin, beta 4; CENTB4; DDEF1ADP-ribosylation factor-directed GTPase-activating protein 1; development and differentiation enhancing factor 1; Development and differentiation-enhancing factor 1; Differentiation-enhancing factor 1; KIAA1249DEF-1; PAG2; PAP; PIP2-dependent ARF1 GAP; ZG14P Asap1|AV239055, DEF-1, Ddef1, PAP, mKIAA1249, s19|ArfGAP with SH3 domain, ankyrin repeat and PH domain1|arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1|130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein|130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein|ADP-ribosylation factor-directed GTPase-activating protein 1|ARF GTPase-activating protein 1|PIP2-dependent ARF1 GAP|development and differentiation-enhancing factor 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Asap1, check out the Asap1 Infographic
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In this infographic, you will see the following information for Asap1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ASAP1 (NM_018482) Human Over-expression Lysate (LS050807)
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