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Product Info Summary
| SKU: | LS023368 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B) |
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Product info
Product Name
ASPP1 (PPP1R13B) (NM_015316) Human Over-expression Lysate
SKU/Catalog Number
LS023368
Size
100 µg
Description
Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)
Storage & Handling
Cite This Product
ASPP1 (PPP1R13B) (NM_015316) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023368)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ASPP1 (PPP1R13B) Human Over-expression Lysates NM_015316.
Western blot validation of overexpression lysate (LS023368) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with PPP1R13B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Ppp1r13b (Source: Uniprot.org, NCBI)
Gene Name
Ppp1r13b
Full Name
Apoptosis-stimulating of p53 protein 1
Weight
119.17kDa
Superfamily
ASPP family
Alternative Names
apoptosis-stimulating protein of p53, 1; ASPP1Protein phosphatase 1 regulatory subunit 13B; KIAA0771apoptosis-stimulating of p53 protein 1; p53BP2-like; p85; protein phosphatase 1, regulatory (inhibitor) subunit 13B Ppp1r13b|A, AI449786, ASPP1, AW545810, Tp53bp2, Trp53bp2, p85|protein phosphatase 1, regulatory subunit 13B|apoptosis-stimulating of p53 protein 1|protein phosphatase 1, regulatory (inhibitor) subunit 13B|transformation related protein 53 binding protein 2|tumor protein p53 binding protein, 2
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Ppp1r13b, check out the Ppp1r13b Infographic
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In this infographic, you will see the following information for Ppp1r13b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ASPP1 (PPP1R13B) (NM_015316) Human Over-expression Lysate (LS023368)
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