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Product Info Summary
| SKU: | LS011285 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7) |
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Product info
Product Name
B4GALT7 (NM_007255) Human Over-expression Lysate
SKU/Catalog Number
LS011285
Size
100 µg
Description
Transient overexpression lysate of xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4GALT7)
Storage & Handling
Cite This Product
B4GALT7 (NM_007255) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011285)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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B4GALT7 Human Over-expression Lysates NM_007255.
Western blot validation of overexpression lysate (LS011285) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with B4GALT7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for B4GALT7 (Source: Uniprot.org, NCBI)
Gene Name
B4GALT7
Full Name
Beta-1,4-galactosyltransferase 7
Weight
37.406kDa
Superfamily
glycosyltransferase 7 family
Alternative Names
b4Gal-T7; beta-1,4-galactosyltransferase 7; Beta-1,4-GalTase 7; beta4Gal-T7; beta4GalT-VII; EC 2.4.1.-; proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7; XGALT-1; XGALT1beta-1,4-galactosyltransferase VII; XGPT1; xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7(galactosyltransferase I) B4GALT7 EDSP1, EDSSLA, EDSSPD1, XGALT1, XGPT, XGPT1 beta-1,4-galactosyltransferase 7 beta-1,4-galactosyltransferase 7|UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7|UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7|UDP-galactose:beta-xylose beta-1,4-galactosyltransferase|beta-1,4-GalTase 7|beta-1,4-galactosyltransferase VII|beta4Gal-T7|beta4GalT-VII|galactosyltransferase I|proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I|xylosylprotein 4-beta-galactosyltransferase|xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7|xylosylprotein beta-1,4-galactosyltransferase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on B4GALT7, check out the B4GALT7 Infographic
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Specific Publications For B4GALT7 (NM_007255) Human Over-expression Lysate (LS011285)
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