Product Info Summary
SKU: | LS010409 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of brain abundant, membrane attached signal protein 1 (BASP1) |
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Product info
Product Name
BASP1 (NM_006317) Human Over-expression Lysate
SKU/Catalog Number
LS010409
Size
100 µg
Description
Transient overexpression lysate of brain abundant, membrane attached signal protein 1 (BASP1)
Storage & Handling
Cite This Product
BASP1 (NM_006317) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010409)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
Click image to see more details
BASP1 Human Over-expression Lysates NM_006317.
Western blot validation of overexpression lysate (LS010409) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with BASP1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for BASP1 (Source: Uniprot.org, NCBI)
Gene Name
BASP1
Full Name
Brain acid soluble protein 1
Weight
22.693kDa
Superfamily
BASP1 family
Alternative Names
BASP1; brain abundant, membrane attached signal protein 1,22 kDa neuronal tissue-enriched acidic protein; brain acid-soluble protein 1; CAP23; CAP-23; CAP23brain acid soluble protein 1; NAP22; NAP-22; NAP22MGC8555; Neuronal axonal membrane protein NAP-22; neuronal tissue-enriched acidic protein BASP1 CAP-23, CAP23, NAP-22, NAP22 brain abundant membrane attached signal protein 1 brain acid soluble protein 1|22 kDa neuronal tissue-enriched acidic protein|brain acid-soluble protein 1|neuronal axonal membrane protein NAP-22|neuronal tissue-enriched acidic protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on BASP1, check out the BASP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BASP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For BASP1 (NM_006317) Human Over-expression Lysate (LS010409)
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No publications found for LS010409
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