BAT4 (GPANK1) (NM_033177) Human Over-expression Lysate

Product Name

BAT4 (GPANK1) (NM_033177) Human Over-expression Lysate

See all GPANK1/BAT4 products

SKU/Catalog Number

LS007918

Size

100 µg

Description

Transient overexpression lysate of HLA-B associated transcript 4 (BAT4)

Storage & Handling

Cite This Product

BAT4 (GPANK1) (NM_033177) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007918)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for GPANK1 (Source: Uniprot.org, NCBI)

Alternative Names

6S54E; ANKRD59Protein G5; Ankyrin repeat domain-containing protein 59; BAT4G patch domain containing 10; D6S54EG patch domain and ankyrin repeats-containing protein 1; G patch domain and ankyrin repeats 1; G patch domain-containing protein 10; G5HLA-B-associated transcript 4; GPATCH10HLA-B associated transcript 4; protein BAT4 GPANK1 ANKRD59, BAT4, D6S54E, G5, GPATCH10 G-patch domain and ankyrin repeats 1 G patch domain and ankyrin repeat-containing protein 1|G patch domain and ankyrin repeats-containing protein 1|G patch domain containing 10|HLA-B-associated transcript 4|ankyrin repeat domain-containing protein 59|g patch domain-containing protein 10|protein BAT4|protein G5

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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