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Product Info Summary
| SKU: | LS023291 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of F-box and WD repeat domain containing 11 (FBXW11), transcript variant 1 |
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Product info
Product Name
beta TRCP2 (FBXW11) (NM_033645) Human Over-expression Lysate
SKU/Catalog Number
LS023291
Size
100 µg
Description
Transient overexpression lysate of F-box and WD repeat domain containing 11 (FBXW11), transcript variant 1
Storage & Handling
Cite This Product
beta TRCP2 (FBXW11) (NM_033645) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023291)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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beta TRCP2 (FBXW11) Human Over-expression Lysates NM_012300.
Western blot validation of overexpression lysate (LS023291) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FBXW11 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
beta TRCP2 (FBXW11) Human Over-expression Lysates NM_033644.
Western blot validation of overexpression lysate (LS023291) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FBXW11 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
beta TRCP2 (FBXW11) Human Over-expression Lysates NM_033645.
Western blot validation of overexpression lysate (LS023291) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FBXW11 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for FBXW11 (Source: Uniprot.org, NCBI)
Gene Name
FBXW11
Full Name
F-box/WD repeat-containing protein 11
Weight
62.091kDa
Alternative Names
beta-transducin repeat-containing protein 2; BTRC2F-box/WD repeat-containing protein 1B; BTRCP2FBW1B; F-box and WD repeat domain containing 11; F-box and WD-40 domain protein 11; F-box and WD-40 domain protein 1B; F-box protein Fbw1b; F-box/WD repeat-containing protein 11; Fbw11; FBXW1BFbw1b; Homologous to Slimb protein; Hos; KIAA0696F-box and WD repeats protein beta-TrCP2 FBXW11 BTRC2, BTRCP2, FBW1B, FBXW1B, Fbw11, Hos, NEDJED F-box and WD repeat domain containing 11 F-box/WD repeat-containing protein 11|F-box and WD repeats protein beta-TrCP2|F-box and WD-40 domain protein 11|F-box and WD-40 domain protein 1B|F-box protein Fbw1b|F-box/WD repeat-containing protein 1B|beta-transducin repeat-containing protein 2|homologous to Slimb protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FBXW11, check out the FBXW11 Infographic
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In this infographic, you will see the following information for FBXW11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For beta TRCP2 (FBXW11) (NM_033645) Human Over-expression Lysate (LS023291)
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