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Product Info Summary
| SKU: | LS080010 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1) |
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Product info
Product Name
BLAP75 (RMI1) (NM_024945) Human Over-expression Lysate
SKU/Catalog Number
LS080010
Size
100 µg
Description
Transient overexpression lysate of RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) (RMI1)
Storage & Handling
Cite This Product
BLAP75 (RMI1) (NM_024945) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS080010)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for Rmi1 (Source: Uniprot.org, NCBI)
Gene Name
Rmi1
Full Name
RecQ-mediated genome instability protein 1
Weight
68.465kDa
Superfamily
RMI1 family
Alternative Names
75 kDa; BLM-associated protein 75 kDa; BLM-associated protein of 75 kDa; chromosome 9 open reading frame 76; FAAP75; FLJ12888; homolog of yeast RecQ-mediated genome instability 1 (RMI1); recQ-mediated genome instability protein 1; RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae); RMI2 Rmi1|4932432N11Rik, C79893|RecQ mediated genome instability 1|recQ-mediated genome instability protein 1|RMI1, RecQ mediated genome instability 1, homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Rmi1, check out the Rmi1 Infographic
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Specific Publications For BLAP75 (RMI1) (NM_024945) Human Over-expression Lysate (LS080010)
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No publications found for LS080010
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