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- Table of Contents
Facts about Phosphatidylcholine translocator ABCB4.
Translocation of PC creates the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent action of bile salts (PubMed:7957936, PubMed:8898203, PubMed:9366571, PubMed:17523162, PubMed:23468132, PubMed:24806754, PubMed:24723470, PubMed:24594635, PubMed:21820390). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to fu rther enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132).
Human | |
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Gene Name: | ABCB4 |
Uniprot: | P21439 |
Entrez: | 5244 |
Belongs to: |
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ABC transporter superfamily |
ABC21; ABCB4; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; EC 3.6.3; EC 3.6.3.44; GBD1; MDR2; MDR3; MDR3MDR2/3; multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 3/multiple drug resistance 3; PFIC-3; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; PGY3; PGY3GBD1
Mass (kDA):
141.523 kDA
Human | |
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Location: | 7q21.12 |
Sequence: | 7; NC_000007.14 (87398988..87476722, complement) |
Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Membrane raft. Cytoplasm. Cytoplasmic vesicle, clathrin-coated vesicle. Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity). Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate- or PPARA-dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of
This Boster Bio: Best Uses for the ABCB4 Marker article will explain how you can utilize the marker to analyze the human immune response. Boster Bio's PICOBAND ELISA Kit is the most popular and with good reason. It offers high-quality detection for the ABCB4 Cytokine, an important marker in the study of neurodegeneration.
The PICOKINE(tm) line of ELISA kits has been developed over twenty years, and has been tested for a variety of samples. Picokine(tm) technology improves the sensitivity of capture antibodies and reduces binding that is non-specific to offer high sensitivity, specificity and low background. The kits are reproducible and include technical support to help you to choose the best one for your needs.
The PICOKINE(tm) ELIISA kit for the ABCB4 marker from Boster Bio has very low background and single-digit picogram sensitive. This ELISA kit is tested at 37°C for a week prior to its introduction to the market. The kit comes with enough reagents for the 96 tests. This ELISA kit for the ABCB4 marker has been approved by regulatory agencies and is widely available in Europe and the United States.
Boster Bio's Picoband(tm), ELISA kit includes Anti-PROM1 (catalog no A01767) that reacts with Human, Mouse or Rat. The antibody is available in lyophilized format and is stable for a month when stored at 4°C. Antibodies are available in a variety of concentrations based on the length of the immunogen.
Picokine(tm) Picokine(tm), one of the Picokine technologies, increases ELISA sensitiveness to the picogram level. Picokine ELISA kit validations are performed using negative and positive samples. Validation images and procedures are available upon request. The Picoband(tm) features Supervision(tm) technology that reduces background and can save up to 30 minutes of IHC time. Picoband comes with high-performance antibodies as well as technical support.
This human fetuin-A PicoKine(tm) ELISA kit was developed by Boster Biological Technology to detect the presence of fetuin-A in cell supernatants. To perform the assay, the sample was mixed with 130 mL of Avidin-Biotin-Peroxidase Complex. The sample was then incubated at 37 degrees Celsius for 30 minutes. After mixing the sample, it was exposed to enzymes, which allowed the antibodies to stain the samples.
All the required reagents are provided in ELISA kits. The principle behind this kind of experiment is to detect molecules using antibodies which are linked to enzymes. The ELISA format may differ, but all of them are based on the same principle of interaction between antibodies and antigens. Picokine(tm) ELISA kits use high-affinity antibody to detect native forms of proteins. Boster's QC department also validates their products against other immunogenic proteins that are similar to theirs and superfamilies.
PMID: 2906314 by van der Bliek A.M., et al. Sequence of mdr3 cDNA encoding a human P-glycoprotein.
PMID: 7893760 by Smit J.J., et al. Characterization of the promoter region of the human MDR3 P- glycoprotein gene.