ABCB4 Antibodies

Phosphatidylcholine translocator ABCB4 (ABCB4)

Energy-dependent phospholipid efflux translocator that functions as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates especially phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes.

Translocation of PC creates the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent action of bile salts (PubMed:7957936, PubMed:8898203, PubMed:9366571, PubMed:17523162, PubMed:23468132, PubMed:24806754, PubMed:24723470, PubMed:24594635, PubMed:21820390). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to fu rther enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132).

Gene Information

Human
Gene Name:	ABCB4
Uniprot:	P21439
Entrez:	5244

Family

Belongs to:
ABC transporter superfamily

Alternative Names

ABC21; ABCB4; ATP-binding cassette sub-family B member 4; ATP-binding cassette, sub-family B (MDR/TAP), member 4; EC 3.6.3; EC 3.6.3.44; GBD1; MDR2; MDR3; MDR3MDR2/3; multidrug resistance protein 3; multiple drug resistance 3; P glycoprotein 3/multiple drug resistance 3; PFIC-3; P-glycoprotein 3; P-glycoprotein-3/multiple drug resistance-3; PGY3; PGY3GBD1

Molecular Weight

Mass (kDA):
141.523 kDA

Genomic Context

Human
Location:	7q21.12
Sequence:	7; NC_000007.14 (87398988..87476722, complement)

Subcellular Localizations

Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Membrane raft. Cytoplasm. Cytoplasmic vesicle, clathrin-coated vesicle. Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity). Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate- or PPARA-dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of

Diseases

• Cholestasis
• Intrahepatic Cholestasis
• Liver Diseases
• Progressive Intrahepatic Cholestasis (disorder)
• Cholestasis Of Pregnancy
• Liver Cirrhosis
• Fibrosis
• Cholelithiasis
• Cholecystolithiasis
• Pregnancy Complications
• Cholangitis
• Pruritus
• Cholangitis, Sclerosing

• Inflammation
• Biliary Cirrhosis
• Malignant Neoplasms
• Injury To Liver
• Hepatic Fibrosis
• Liver Carcinoma
• Liver Damage

Pathways

• Transport
• Secretion
• Excretion
• Pathogenesis
• Gallstone Formation
• Drug Resistance
• Localization
• Cholesterol Transport
• Reverse Cholesterol Transport
• Cholesterol Efflux
• Lipid Transport
• Lipid Homeostasis
• Auxin Transport

• Phospholipid Transport
• Auxin Efflux
• Drug Transport
• Cell Proliferation
• Cholesterol Homeostasis
• Reverse Transcription
• Translation

PTMs

• Methylation
• Demethylation
• Reduction

• Acetylation
• Oxidation
• Ribosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ABCB4

Boster Bio: Best Uses of the ABCB4 Marker

This Boster Bio: Best Uses for the ABCB4 Marker article will explain how you can utilize the marker to analyze the human immune response. Boster Bio's PICOBAND ELISA Kit is the most popular and with good reason. It offers high-quality detection for the ABCB4 Cytokine, an important marker in the study of neurodegeneration.

Boster Bio's PICOKINE(tm) ELISA kit

The PICOKINE(tm) line of ELISA kits has been developed over twenty years, and has been tested for a variety of samples. Picokine(tm) technology improves the sensitivity of capture antibodies and reduces binding that is non-specific to offer high sensitivity, specificity and low background. The kits are reproducible and include technical support to help you to choose the best one for your needs.

The PICOKINE(tm) ELIISA kit for the ABCB4 marker from Boster Bio has very low background and single-digit picogram sensitive. This ELISA kit is tested at 37°C for a week prior to its introduction to the market. The kit comes with enough reagents for the 96 tests. This ELISA kit for the ABCB4 marker has been approved by regulatory agencies and is widely available in Europe and the United States.

PICOBAND(tm), ELISA kit

Boster Bio's Picoband(tm), ELISA kit includes Anti-PROM1 (catalog no A01767) that reacts with Human, Mouse or Rat. The antibody is available in lyophilized format and is stable for a month when stored at 4°C. Antibodies are available in a variety of concentrations based on the length of the immunogen.

Picokine(tm) Picokine(tm), one of the Picokine technologies, increases ELISA sensitiveness to the picogram level. Picokine ELISA kit validations are performed using negative and positive samples. Validation images and procedures are available upon request. The Picoband(tm) features Supervision(tm) technology that reduces background and can save up to 30 minutes of IHC time. Picoband comes with high-performance antibodies as well as technical support.

PICOKINE(tm), ELISA kit

This human fetuin-A PicoKine(tm) ELISA kit was developed by Boster Biological Technology to detect the presence of fetuin-A in cell supernatants. To perform the assay, the sample was mixed with 130 mL of Avidin-Biotin-Peroxidase Complex. The sample was then incubated at 37 degrees Celsius for 30 minutes. After mixing the sample, it was exposed to enzymes, which allowed the antibodies to stain the samples.

All the required reagents are provided in ELISA kits. The principle behind this kind of experiment is to detect molecules using antibodies which are linked to enzymes. The ELISA format may differ, but all of them are based on the same principle of interaction between antibodies and antigens. Picokine(tm) ELISA kits use high-affinity antibody to detect native forms of proteins. Boster's QC department also validates their products against other immunogenic proteins that are similar to theirs and superfamilies.