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- Table of Contents
Facts about Tyrosine-protein kinase ABL1.
Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility via phosphorylation of key regulators of these processes like BCAR1, CRK, CRKL, DOK1, EFS or NEDD9.
Human | |
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Gene Name: | ABL1 |
Uniprot: | P00519 |
Entrez: | 25 |
Belongs to: |
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protein kinase superfamily |
Abelson murine leukemia viral oncogene homolog 1; ABL; ABL1; bcr/abl; BCR-ABL1; c-abl oncogene 1, non-receptor tyrosine kinase; c-abl oncogene 1, receptor tyrosine kinase; cAbl; c-Abl; EC 2.7.10; EC 2.7.10.2; JTK7; JTK7bcr/abl; p150bcr/c-abl oncogene protein; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene homolog 1; v-abl
Mass (kDA):
122.873 kDA
Human | |
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Location: | 9q34.12 |
Sequence: | 9; NC_000009.12 (130713881..130887675) |
Widely expressed.
Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity).; [Isoform IB]: Nucleus membrane; Lipid-anchor. The myristoylated c-ABL protein is reported to be nuclear.
You may be wondering how to make the most of your boster bio experiments. You've probably wondered about Steven Boster, but now you know. His biography and history are fascinating, and you're about to discover some of the most popular uses of the ABL1 marker. Read on to discover more about Boster Bio and the ABL1 marker. In addition to learning about his background, this article will give you a brief overview of Immunofluorescence and Super Vision Detection Kits.
In Ana Boster Bio: Best Uses of the ABL1 Marker, we discuss the latest research and its application to cancer. This biomarker is derived from the gene ABLR, which is involved in the development of the human BRCA gene. Its main purpose is to regulate the growth of tumor cells in the body. There are many applications of this gene, including its use to track the progression of cancer cells.
Boster Bio is a company that makes research antibodies and ELISA kits to detect ABL1 and related proteins. Their products have been validated for a variety of research applications and can detect biomarkers in cancer, development biology, neurosciences, inflammation, and more. Each of their antibodies is highly specific and can detect biomarkers at a picogram level. Their ELISA kits are available through tebu-bio.
After decades of developing ELISA kits, Steven Boster is now a renowned biotechnology business owner. In 1993, he created his first product, earning the nickname "he who converts science in the lavatory". Since then, Boster has developed hundreds of primary antibodies, including some of the most sensitive and specific ELISAs on the market. His business grew to be one of the largest catalog antibody companies in China by the late 1990s.
The ABL1 marker is a cytotoxic T cell subset and is present on 20 to 35% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and other bone marrow cells. The CD8 antigen is a restricted material and should only be used for research purposes. It should never be used for diagnostic procedures or resold without the permission of the manufacturer.
The ABL1 marker can be used in numerous applications and can be found in a wide range of secondary antibody conjugates. This system enables dual labeling of specimens, which allows for more questions to be asked of each sample. Moreover, the dual labeling makes it possible to get context data as well as robust answers. Using the ABL1 marker is an excellent way to detect certain cancer cells in their early stages.
The Super Vision two-step Immunohistochemistry Assay Kit is a highly advanced immunohistochemistry detection system that offers superior sensitivity, speed, and ease of use. The system utilizes avidins conjugated to signal molecules (typically, Horseradish Peroxide, HRP), allowing researchers to target proteins of interest while maintaining low background levels. These features make the Super Vision immunohistochemistry assay kit a top choice for immunohistochemical analysis.
BCR-ABL1 is a tyrosine kinase that regulates the activation of RAC guanosine triphosphatases (GTPases). These GTPases integrate growth factors, signal chemokines, and adhesion receptors. BCR-ABL1 promotes cell proliferation by activating a variety of effectors. One of these effectors, STAT5, mediates BCR-ABL1 kinase-mediated transformation. Inhibition of this gene by a specific RAC inhibitor is effective in abrogating this process.
BCR-ABL1 is composed of a series of functionally distinct domains. These domains are joined by the "Cap" region of the protein, which contains a C14 myristoyl moiety. Type 1a and BCR-ABL1 kinase are two isoforms of the same protein, and the latter is expressed at higher levels than type 1a. Both ABL1 isoforms are structurally identical, but one has a myristoyl moiety covalently linked to the N terminus.
A patient with BCR-ABL1 amplification showed enrichment in the promoter region of the gene. RNA sequencing using a BCR-ABL1-positive K562 cell line indicated enrichment of the promoter region of BCR-ABL1. MYC-R1 and MYC-R2 were used as positive controls. They are compared to the corresponding negative controls.
The activation loop of the BCR-ABL1 kinases is depicted in the protein structure. It consists of an activation loop, an Src-like inactive conformation, and an aC helix. Several ionic interactions are indicated by dotted lines. SH2-KD contains two mutanted variants: T231R and DFG motif. The mutation T231R enhances the stimulating effect of the SH2 domain.
The mutants Ala-108 and Glu-108 replaced the Thr-108 residue in apoptin with alanine and glutamine, respectively. These mutations mimicked phosphorylated apoptin while being non-toxic to cells. Moreover, they retained the ability to interact with BCR-ABL1p210. These mutants have also been shown to inhibit the growth of tumor cells by blocking the activity of BCR-ABL1p210.
PMID: 3021337 by Shtivelman E., et al. Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene.
PMID: 2687768 by Fainstein E., et al. Nucleotide sequence analysis of human abl and bcr-abl cDNAs.