Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1)

Catalyzes the desaturation of acyl-CoAs into 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length.

Isoform 2 is active against a much broader range of substrates and reveals activity towards quite long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 from 16-hydroxy- palmitoyl-CoA and is 25% more active from 1,16-hexadecanodioyl- CoA.