Product Info Summary
| SKU: | M03054 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-ACOX1 Antibody
SKU/Catalog Number
M03054
Size
80 µl
Description
Boster Bio Anti-ACOX1 Antibody (Catalog # M03054). Tested in IF, IHC-P, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ACOX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03054)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
153CT43.1.1
Isotype
IgG1
Immunogen
This ACOX1 monoclonal antibody is generated from mouse immunized with ACOX1 recombinant protein.
Reactive Species
M03054 is reactive to ACOX1 in Human
Calculated molecular weight
74.4 kDa
Background of ACOX1
ACOX1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03054 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:4000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of ACOX1 Antibody with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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All lanes : Anti-ACOX1 Antibody at 1:4000 dilution
Lane 1: Human liver lysate
Lane 2: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 74 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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ACOX1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma
followed by peroxidase conjgation of the secondary antibody and DAB staining. This data demonstrates the use of the ACOX1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Specific Publications For Anti-ACOX1 Antibody (M03054)
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