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- Table of Contents
Facts about AF4/FMR2 family member 4.
In the event of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. .
Human | |
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Gene Name: | AFF4 |
Uniprot: | Q9UHB7 |
Entrez: | 27125 |
Belongs to: |
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AF4 family |
AF4/FMR2 family, member 4; AF5Q31AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Major CDK9 elongation factor-associated protein; MCEFALL1 fused gene from 5q31; MGC75036; Protein AF-5q31
Mass (kDA):
127.459 kDA
Human | |
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Location: | 5q31.1 |
Sequence: | 5; NC_000005.10 (132875381..132963655, complement) |
Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain.
Nucleus. Associates to transcriptionally active chromatin but not at snRNA genes.
If you have ever wondered how to use the AntiAF4/FMR2 4 (AF4/FMR2) gene probe, here are some tips. Learn about the high affinity primary antibodies and their applications. Also, check out the gene infographics. Boster's gene Infographics will give you a better understanding about the gene's functions.
Anti-AF4/FMR2 four (AF4/FMR2) antibody can be used to detect AFF4 proteins in biological samples. This monoclonal antibody is a ready-to-use microwell, strip plate ELISA kit that detects native AFF4 protein. It is highly reactive and useful in detecting AFF4 proteins.
This antibody is highly effective. It can be used in breast cancer detection of AF4/FMR2 member 4 (AF4) marker. This antibody is extremely sensitive and can detect biomarkers of cancer at picogram levels. Boster Bio also produces antibodies and immunological substances. You can purchase its products at bosterbio or tebubio.
One of the best sources of rabbit monoclonal antibodies for the Diagnostics and Therapeutics industry is Boster Bio. Boster Bio produces clones with the highest affinity for target antigens as well as their associated ligands. Its PCD platform employs proprietary chemistry, flow cytometry, and proprietary chemistry to prevent B cells releasing antibodies and keep them on their cell membranes. Boster then incubates splenocytes with flurochrome-conjugated antigens to detect the antibody clones that have the highest brightness.
Boster's ELISA kit design allows users to detect a variety substances in biological samples. This includes hormones, small molecules and inflammatory cytokines. HSP70 and other compounds that can affect human health. Boster ELISA kit are suitable for a variety sample types and contain enough reagents to run 96 tests.
Scientists must first create monoclonal monoclonal antibodies from their host immune system. These antibodies contain complementarity-determining regions (CDRs) that enable them to detect specific antigens. Wikipedia provides more information on these regions. Scientists will perform an ELISA titration testing after selecting the antibodies. The subcloning process for the positive supernatants is limiting-dilution. Final testing will be done on the selected antibodies.
Enzyme-linked immunosorbent assays work similar to other immunoassay technologies. ELISAs use specific antibodies that bind to target antigens to detect the resulting antigens. High-affinity antibody coatings are used to increase the assay's specificity. Boster Bio has become a trusted partner for scientists around the world, thanks to their expertise in coating plates with these antibodies.
Secondary antibodies are also used for testing the specificity of primary antibodies. They bind the target protein, then cross-react with secondary antibodies to form a colored compound. The colored substance is a band of protein. The density of the target antibody within the tissue/cell is represented by the bands. This measurement is important in determining whether the primary antibody is effective. If it isn’t, the secondary anti-body may not work correctly and the results might be misleading.
The AFF4 marker can be used for many applications. It is used to target acquisitions of data and for exporting subsets. AFF4, the predecessor to AFF4, offered compression and started to store metadata in the image. Nevertheless, AFF4 is not perfect. AFF4L-L isn't compatible with all types data sources. This marker may not be compatible with large, unstructured files.
The AFF4 marker can be used to preserve forensic integrity. Its file format is open source and vendor-neutral. It allows data export and movement between tools. It helps to preserve data integrity. It is a good choice to use for forensics since it supports multiple file format. It is also an open standard which means that any program can use it.
AFF4 is also a marker that promotes osteogenic differentiation. This was achieved by infecting DFCs stably with lentivirus expressing AFF4 or GFP. Both gene types showed a similar effect in bone-forming cells, and qRT-PCR and western blot analyses demonstrated that AFF4 overexpression induced a pronounced increase in ALP staining and activity. Overexpression also promoted expression of BGLAP and DLX5, which are key markers for osteogenic differentiation.
AFF4 has a second important function in cancer stem cell research. Studies have shown high levels AFF4 in cancer stem cells. AFF4-deficient cells had a lower ability to spread tumors than control-expressing mice. Additionally, m6A/m6A-modified cells are less susceptible to tumors than normal AFF4-expressing cells.
Gene Infographics provides information about genes, including the AFF4 marker. Genes are presented in an easy-to-understand format with basic information about each one. There are several gene infographics for both human and mouse genomes. The site features a search tool that will allow you to quickly locate the gene you're interested in. There are many publications that associate the AFF4 gene.
CdLS and CHOPS have a similar transcriptional profile. This indicates that the AFF4 gene may be regulated in both conditions. The top 250 genes are also represented with the same color, and they are both shown in the same way. The bottom plot shows which genes are controlled by this marker and which are not. This is done with volcano plots. The genes that are regulated by AFF4 are depicted with similar shapes and sizes.
Recent evidence has shown that AFF4 protein accumulates at the transcriptomes in CHOPS-syndrome patients. Its accumulation was not correlated with RNA-seq and RNAP2 Se2ph, suggesting that AFF4 protein regulates the expression of a subset genes. Luo et. al. have demonstrated that this is the truth. Luo and colleagues demonstrated that knockdowns in AFF4 reduced gene expression.
PMID: 10588740 by Taki T., et al. AF5q31, a newly identified AF4-related gene, is fused to MLL in infant acute lymphoblastic leukemia with ins(5;11)(q31;q13q23).
PMID: 12065898 by Estable M.C., et al. MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b- associated protein.