Azurocidin Antibodies

Azurocidin (AZU1)

Binds heparin. The cytotoxic action is limited to a lot of species of Gram- negative bacteria; this specificity could be explained by a strong affinity of the very fundamental N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.

It may play a role in mediating recruitment of monocytes in the next wave of inflammation. Has antibacterial action against the Gram-nagative bacterium P.

Gene Information

Human
Gene Name:	AZU1
Uniprot:	P20160
Entrez:	566

Family

Belongs to:
peptidase S1 family

Alternative Names

AZAMP; AZU; AZU1; azurocidin 1; Azurocidin; CAP37; CAP37Heparin-binding protein; cationic antimicrobial protein 37; HBP; HBPCationic antimicrobial protein CAP37; HUMAZUR; NAZC; neutrophil azurocidin

Molecular Weight

Mass (kDA):
26.886 kDA

Genomic Context

Human
Location:	19p13.3
Sequence:	19; NC_000019.10 (827837..832018)

Subcellular Localizations

Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side. Localizes to azurophil granules of neutrophil granulocytes. Also called primary granules, these specialized lysosomes of the neutrophil formed early during promyelocyte development store antibacterial proteins and peptides.

Diseases

• Lung Diseases
• Chronic Obstructive Airway Disease
• Lung Diseases, Obstructive
• Myocardial Ischemia
• Dyslipidemias
• Bronchitis, Chronic
• Malignant Neoplasm Of Lung
• Leukemia, Myelocytic, Acute
• Peripheral Vascular Diseases
• Cardiovascular Diseases
• Malignant Neoplasms
• Myeloid Leukemia
• Pulmonary Emphysema

• Bacterial Infections
• Tissue Damage
• Cerebrovascular Disorders
• Atherosclerosis
• Diabetes Mellitus
• Hypertensive Disease
• Heart Diseases

Pathways

• Transport
• Dna Methylation
• Protein Transport
• Ion Homeostasis
• Cellular Ion Homeostasis
• Methylation
• Chemotaxis
• Coagulation
• Hemostasis
• Lymphocyte Activation
• Complement Activation
• Opsonization

• Myeloid Cell Differentiation
• Drug Resistance
• Cell Differentiation
• Chromatin Organization
• Interphase
• Pathogenesis
• Chromatin Remodeling

PTMs

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/AZU1

Best Uses Of The AZU1 Marker

There are many ways to measure AZU1 levels in human samples. Among these methods are Tissue culture, Immunohistochemistry, and ELISA. To know what each method does, refer to the following sections. Below are the top methods to test AZU1 levels of protein in human samples. However, the exact method to determine the protein levels differs from one lab to the next.

Bone Marrow

The AZU1 marker that is found in neutrophils, is used as a biomarker used to identify neutrophils. It can also help determine the progenitors of neutrophils. In the present study, eNeP were isolated from bone marrow neutrophils. They were characterized by the expression of a range of markers on the surface that are typical of progenitors. This observation was not confirmed by the expression of CD11b or CD101 markers on these cells. eNeP is the only progenitor of CD66b+ bone marrow neutrophils.

The AZU1 marker was initially created to identify proliferating neutrophils. However until recently, no research had identified the progenitor cells. In fact, a study by Dinh and co. showed that neutrophils marked by CD117+CD71+ are increased in patients with lung cancer. This study demonstrates that the progenitors of early neutrophils that are associated with cancer are more abundant in human bone.

Other studies have demonstrated that CD71 positivity is present in eNeP as well as mature neutrophils. These findings suggest that eNeP may be the earliest neutrophils' bone marrow progenitors. While they only comprise 0.14 percent of the bone marrow neutrophil population their eNeP expression pattern is consistent with their diffusion map trajectory. Additionally, eNePs contain specific surface markers that are characteristic of early neutrophil progenitors.

A recent study has revealed the differences between pre- and post-natal neutrophils in the study described. A cluster analysis of CyTOF results revealed that pre-Neus were proliferating bone marrow neutrophils that had a number of transcription factors related to the development of neutrophils. These cells were found close to CXCL12-producing retinal cells. The results also showed that preNeus were a subset of CD15+CD66b+ neutrophils.

Tissue culture

Boster Bio's AZU1 antibody is a multi-functional antibody that detects FGFR2 tissue cultures. This antibody against AZU1 reacts with human, mouse, and rat tissues and is validated in multiple platforms for specificity and affinity. Boster's firm rewards the first reviewers of its products with product credits, which are then accessible to all scientists.

Immunohistochemistry

Boster Bio has developed research antibodies and ELISA kits to detect a variety of biomarkers in cancer, neurosciences inflammation, and the development of biology. These kits are sensitive to the sensitivity of a picogram. The Boster biomarker reagents can be found in tebu-bio. The AZU1 antibody is part of the Human Immunoglobulin superfamily.

Boster Bio Anti-Azurocidin/AZU1 has been included in the Picoband(tm). It has been tested for use with ELISA, Flow Cymetry, and IHC-P. The antibody reacts with mouse, human and rats cells. If you are looking for the AZU1 antibody to study a specific gene, the lysis buffer provided by Boster Bio is a good option. It reduces the intensity of cross-linking and ensures a high quality antibody.

ELISA

The Boster Bio Azu1 ELSA is an accurate and sensitive kit to test soluble protein. The simple protocol has been designed to enhance the performance of your experiment. The protocol provides you with step-by step instructions on how to conduct the ELISA which includes preparation of the sample, the concentration of the antibody and blocking buffer. The ELISA optimization guide offers a step-by–step explanation of the entire procedure as well as a useful reference guide.

The human AZU1 ELISA kit has 96 wells that measure Human AZU1 protein in plasma or serum. It has an sensitivity of 10 pg/ml and there is no cross-reactivity. The kit can be used to analyze the expression levels of AZU1 in human cells and in animal models. The kit also includes a blocking peptide to the CAP7 protein. This can be purchased at an additional cost.