DVL2 Antibodies

Segment polarity protein dishevelled homolog DVL-2 (DVL2)

Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C- terminus of frizzled family members and transducing the Wnt signal to down-stream effectors.

Promotes internalization and degradation of frizzled proteins upon Wnt signaling. .

Gene Information

Human
Gene Name:	DVL2
Uniprot:	O14641
Entrez:	1856

Family

Belongs to:
DSH family

Alternative Names

dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2 (Drosophila); Dishevelled2; Dishevelled-2; DSH homolog 2; DVL2; segment polarity protein dishevelled homolog DVL-2

Molecular Weight

Mass (kDA):
78.948 kDA

Genomic Context

Human
Location:	17p13.1
Sequence:	17; NC_000017.11 (7225341..7234544, complement)

Subcellular Localizations

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Cytoplasmic vesicle. Nucleus. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta (By similarity). Interaction with FOXK1 and FOXK2 induces nuclear translocation (PubMed:25805136).

Diseases

• Malignant Neoplasms
• Neoplasms
• Tissue Adhesions
• Cell Transformation, Neoplastic
• Colorectal Cancer
• Cell Invasion
• Crest Syndrome
• Malignant Neoplasm Of Stomach
• Malignant Neoplasm Of Breast
• Carcinoma
• Mammary Neoplasms
• Ulcer
• Congenital Abnormality

• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Lung
• Fracture
• Neoplasm Metastasis
• Malignant Tumor Of Colon
• Colorectal Neoplasms
• Carcinogenesis

Interacting Proteins

• APC
• AXIN1
• DACT1
• DIXDC1
• LRRK2

• Pard6a
• RIPK4
• Smurf2
• TIFA
• TP53

• TRIM29
• WWTR1
• ZNF165

Pathways

• Localization
• Cell Migration
• Cell Proliferation
• Convergent Extension
• Neural Tube Closure
• Tube Closure
• Cell Cycle
• Endocytosis
• Cell Growth
• Cell Adhesion
• Segmentation
• Rna Interference
• Cell Motility

• Neurogenesis
• Mitosis
• Autophagy
• Clathrin-mediated Endocytosis
• Transposition
• Osteoblast Differentiation
• Lung Development

PTMs

• Phosphorylation
• Cleavage

• Methylation
• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DVL2

Best Uses Of The DVL2 Marker

This article outlines the main factors to consider when purchasing antibodies. Boster Bio produces high-affinity primary antibody. These antibodies have been well-cited for over 25 years and have been validated in ELISA, Immunohistochemistry, and Western Blotting. These antibodies are guaranteed to work, as they are highly specific for this protein. Boster primary antibodies also come from purified goat anti–mouse antigen.

DVL2 Marker

This study evaluated the effects of knockdown of the DVL2 gene on colony forming capacity of pancreatic cells. The DVL2 gene knockdown significantly suppressed colony forming capacity of pancreatic cells, while it inhibited the growth of PDAC cells. The results of this study were encouraging, but further research is needed to determine the exact oncogenic role of DVL2 genes and the molecular mechanism that causes tumors.

The optimal use of this gene is currently unknown. It has been associated with a positive prognostic signal in the disease. DVL2 protein expression is associated in PDAC cells as well as tissues with a positive prognostic signal. If your PDAC tumor has metastatic status, then DVL2 expression can be used as a marker to help you identify it.

The data were analyzed with the open Gene Expression Omnibus database (GEO). It contained 36 pairs of normal tissue and tumor samples. The PDAC tissues had significantly higher levels of DVL2 than the adjacent normal samples. IHC staining was also performed on 97 PDAC tissues as well as 85 normal samples. 64 of 97 PDAC cells contained high levels of DVL2 genes.

These findings highlight DVL2's importance in pancreatic disease. It could also be a therapeutic target for pancreatic carcinoma. In vivo, DVL2 knockdown inhibits PC proliferation and metastasis. The researchers say that knockdown of the DVL2 gene might be beneficial for patients with pancreatic cancer. The National Natural Science Foundation of China grant 81900772, and the Kangda College of Nanjing Medical University contributed to the research.

The DVL2 Gene is involved in Wnt Signaling Regulation and is associated to a variety of pathways. It regulates gene transcription through the activation of the Wnt/b-catenin pathway. DVL2 promotes Wnt target gene transcription by binding b–catenin to the c-myc enhancer. Moreover, knockdown DVL2 also inhibits the phosphorylation GSK3b that binds DVL2.

The DVL2 signal transducing protein is a gene that encodes a signal-transducing protein. It participates in both noncanonical and canonical WNT signaling. It plays a vital role in pancreatic growth, islet function and insulin secretion. Normal function requires that DVL2 expression be within a specified range. DVL2 can be used as a therapeutic target due to its abnormally high expression. The death of cells is caused by DVL2 knockdown.

High-affinity primary antibodies

The absence IgD, a cellular molecule required for the transition of a primary autoreactive reaction to a secondary antibody-specific response, slows down and impedes the production of high affinity primary antibodies in mice. A prolonged autoimmune condition is caused by a deficiency of IgD. IgD is responsible for the production of protective high-affinity IgM in humans.

CD146 was shown to interact endogenously with Dvl in experiments involving DVL2 protein. This interaction was also confirmed in coIP experiments, where HUVEC cell lines were treated with Wnt5a CM. A cytokine known for promoting Dvl2 phosphorylation, Wnt5a CM was used to treat them. When cells co-express CD146 or Dvl2, Wnt5a causes the inhibition of this interaction, which results in a blockage to Wnt5a-induced Dvl2phosphorylation.

For efficient recombination and fragmentation of antibodies, it is vital that B cells have flexibility in the DVL2 marker. This molecule is crucial for primary immune responses. It is also essential for maturation B-lymphocytes. High-affinity primary antibodies utilizing the DVL2 marker have the potential to provide highly specific antigen-specific immune responses.

As we have already mentioned, the KD value for each target's antibody is related to its affinity. The lower the KD, the higher its affinity. The data were gathered at UC Davis. Abcam scientists reviewed the data and validated it. The results were encouraging. This method will allow researchers better target HIV/AIDS patients. And this is just one of the methods that have improved patient care.

Affinity is the strength of binding between a single molecule and a specific ligand. It is usually measured using the equilibrium dissociation constant (KD). KD is a reverse process. The rate that affinity binding takes place is proportional the reactants' concentrations as well as their dissociation rate into the products. KD can also be defined as the rate at which two or more species react. The higher the affinity of an antibody, the lower the KD value.

After determining the optimal bindingaffinities of the antibodies, they were tested in the spleen with polyclonal rat IgG–UNLB as a control. They were also tested for biotin-conjugated propeptides. High-affinity primary antibodies using the DVL2 marker were found to have high-affinity binding properties for both the Fc and Wnt5a receptors.

The IgD class BCR is crucial for the affinity maturation insulin-IgM. We used peptide ratio ELISA as an instrument to measure insulin-specific IgM affinities over different days. We found that WT mice achieved high-affinity insulin IgM on day 52. IgD deficient mice didn't reach high-affinity insulin IgM until 72. Both groups of mice displayed clinical signs that indicated diabetes.