Probable E3 ubiquitin-protein ligase HERC1 (HERC1)

Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP.

Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also function as a E3 ubiquitin- protein ligase which accepts ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.