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- Table of Contents
ICAM1 antibody (M00171-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of ICAM1 using anti-ICAM1 antibody (M00171-1).
ICAM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ICAM1 Antibody (M00171-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ICAM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ICAM1 Antibody (M00171-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for ICAM1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ICAM1 Western Blot GuideBundle Save
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody
ICAM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
ICAM1 antibody was positively detected by WB in:
human HepG2 whole cell, human SIHA whole cell, human K562 whole cell, human A431 whole cell, human Romas whole cell, human Hela whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ICAM1 antibody was positively detected by IHC in:
human placenta tissue, human lung cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody
View all ICAM1 Antibodies
View all ICAM1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DOD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ICAM1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
57.8 kDa
Cite This Product
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171-1)
Protocols
Western Blot protocol for ICAM1 antibody
Immunohistochemistry (IHC) protocol for ICAM1 antibody
Publications
Application:
Species:
Device surface-functionalized ac-SDKP nanoassemblies accelerate endothelial recovery through dual anti-inflammatory and endothelial homeostatic mechanisms
Published: 2026-01-06
Journal: Biomaterials Advances
Application: IF
Impact Factor: 6.00
Species: Human
PMID: 41529529
I4, a synthetic anti-diabetes agent, attenuates atherosclerosis through its lipid-lowering, anti-inflammatory and anti-apoptosis properties
Published: 2016-10-08
Journal: MOLECULAR AND CELLULAR ENDOCRINOLOGY
Application: IHC
Impact Factor: 3.86
Species: Mouse
PMID: 27725191
Anti-Inflammatory Effect of the Blueberry Anthocyanins Malvidin-3-Glucoside and Malvidin-3-Galactoside in Endothelial Cells
Published: 2014-08-21
Journal: MOLECULES
Application: WB
Impact Factor: 2.10
Species: Human
PMID: 25153881
Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway
Published: 2014-03-18
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 2.11
Species: Human
PMID: 24721321
Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-α and its possible role in wound healing
Published: 2009-03-13
Journal: WOUND REPAIR AND REGENERATION
Application: ICC
Impact Factor: 2.15
Species: Rat
PMID: 19320886
Aspirin protected against endothelial damage induced by LDL: role of endogenous NO synthase inhibitors in rats.
Published: 2004-12-01
Journal: ACTA PHARMACOLOGICA SINICA
Impact Factor: 0.88
PMID: 15569408
Review
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Product Promise
We promise Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00171-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026