Rat ICAM-1 ELISA Kit PicoKine™

Rat ICAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. Cited in 22 publication(s).

Product Info Summary

SKU: EK0372
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat ICAM-1 ELISA Kit PicoKine™

See all ICAM-1/CD54 products

SKU/Catalog Number

EK0372

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat ICAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat ICAM-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0372)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q28-T493

Sensitivity

<2 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0372 is reactive to ICAM1 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0372 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0372-CAP Anti-Rat ICAM1 Pre-coated 96-well strip microplate 1
EK0372-ST Rat ICAM1 Standard 2 vials, 10 ng/tube
EK0372-DA Rat ICAM1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0030.0820.1400.2980.5530.9671.4272.142

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat ICAM-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5123178751242855
Standard deviation3.6714.5542.494.2316.2151.3
CV (%)7.2%6.7%5.4%8.3%6.7%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ICAM-1/CD54 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 151474956503.346.6%
Sample 223122727524724518.867.6%
Sample 378776186279780137.184.6%
*number of samples for each test n=16.

Gene/Protein Information For ICAM1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05362

Gene ID

3383

Gene Name

ICAM1

Full Name

Intercellular adhesion molecule 1

Weight

57.825kDa

Superfamily

immunoglobulin superfamily

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ICAM1, check out the ICAM1 Infographic

ICAM1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ICAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0372 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ying-nan Ju,Qi-hang Tai,Guang-xiao Xu,Xiao-qing Zhao,Hai-bin Sun,Wei Gao,"Diannexin Can Ameliorate Acute Respiratory Distress Syndrome in Rats by Promoting Heme Oxygenase-1 Expression",Mediators of Inflammation,vol.2021,Article ID 1946384,10 pages,2021.https://doi.org/10.1155/2021/1946384
Species: Rat
EK0372 usage in article: APP:ELISA, SAMPLE:BALF AND SERUM, DILUTION:NA

He C,Su C,Zhang W,Zhou Q,Shen X,Yang J,Shi N.Modulatory Potential of LncRNA Zfas1 for Inflammation and Neuronal Apoptosis in Temporal Lobe Epilepsy.Yonsei Med J.2021 Mar;62(3):215-223.doi:10.3349/ymj.2021.62.3.215.PMID:33635011.
Species: Human,Rat

Wang Q, Xu GX, Tai QH, Wang Y. Lipoxin A4 Reduces Ventilator-Induced Lung Injury in Rats with Large-Volume Mechanical Ventilation. Mediators Inflamm. 2020 Nov 22;2020:6705985. doi: 10.1155/2020/6705985. PMID: 33299377; PMCID: PMC7704204.
Species: Rat
EK0372 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Alpha 1-antitrypsin ameliorates ventilator-induced lung injury in rats by inhibiting inflammatory responses and apoptosis

Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction

Esculin improves dyslipidemia, inflammation and renal damage in streptozotocin-induced diabetic rats

Urocortin induced expression of COX-2 and ICAM-1 via corticotrophin-releasing factor type 2 receptor in rat aortic endothelial cells

Chen C, Chen W, Li Y, Dong Y, Teng X, Nong Z, Pan X, Lv L, Gao Y, Wu G. Oncotarget. 2017 Dec 4;8(67):111522-111534. doi: 10.18632/oncotarget.22869. eCollection 2017 Dec 19. Hyperbaric oxygen protects against myocardial reperfusion injury via the i...

Gu R, Ding X, Tang W, Lei B, Jiang C, Xu G. Front Pharmacol. 2018 Apr 6;9:331. doi: 10.3389/fphar.2018.00331. eCollection 2018. A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal M

Yang B, Gao P, Wu X, Yu J, Li Y, Meng R, Li Y, Yan J, Jin X. Exp Ther Med. 2017 Sep;14(3):1975-1982. doi: 10.3892/etm.2017.4774. Epub 2017 Jul 11. Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury...

Jiang Jx, Zhang Sj, Xiong Yk, Jia Yl, Sun Yh, Lin Xx, Shen Hj, Xie Qm, Yan Xf. Plos One. 2015 Jun 2;10(6):E0128278. Doi: 10.1371/Journal.Pone.0128278. Ecollection 2015. Eets Attenuate Ox-Ldl-Induced Ltb4 Production And Activity By Inhibiting P38 M...

Zhang Yf, Yang X, Zhang Yj, Sun Yl, Zou Xl, Kong Qy, Dong Xq, Ye Xq, Yu Xq. Am J Nephrol. 2006;26(6):602-11. Epub 2006 Dec 20. Peroxisome Proliferator-Activated Receptor-Gamma Is Expressed By Rat Peritoneal Mesothelial Cells: Its Potential Role In...

Xiang M, Qian Zy, Zhou Ch, Liu J, Li Wn. J Ethnopharmacol. 2006 Aug 11;107(1):25-31. Epub 2006 Mar 10. Crocetin Inhibits Leukocyte Adherence To Vascular Endothelial Cells Induced By Ages.

Tian Xx, Wang Bl, Cao Yz, Zhong Yx, Tu Yy, Xiao Jb, He Qf, Zhai Ln. Chin J Integr Med. 2015 Mar;21(3):229-33. Doi: 10.1007/S11655-013-1513-8. Epub 2013 Oct 19. Comparison Of Protective Effects Of Safflor Injection And Extract Of Ginkgo Biloba On L...

Yang Py, Rui Yc, Lu L, Li Tj, Liu Sq, Yan Hx, Wang Hy. Life Sci. 2005 Sep 30;77(20):2529-39. Time Courses Of Vascular Endothelial Growth Factor And Intercellular Adhesion Molecule-1 Expressions In Aortas Of Atherosclerotic Rats.

Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L....

Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients?

Jiang Jx, Zhang Sj, Liu Yn, Lin Xx, Sun Yh, Shen Hj, Yan Xf, Xie Qm. Eur J Pharmacol. 2014 Mar 15;727:43-51. Doi: 10.1016/J.Ejphar.2014.01.045. Epub 2014 Jan 31. Eets Alleviate Ox-Ldl-Induced Inflammation By Inhibiting Lox-1 Receptor Expression In...

Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.

Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

Jing L, Wang Jg, Zhang Jz, Cao Cx, Chang Y, Dong Jd, Guo Fy, Li Pa. J Inflamm (Lond). 2014 Nov 5;11(1):35. Doi: 10.1186/S12950-014-0035-2. Ecollection 2014. Upregulation Of Icam-1 In Diabetic Rats After Transient Forebrain Ischemia And Reperfusion...

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4 Customer Q&As for Rat ICAM-1 ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-07-05

Question

Q: if the enzyme conjugated CD54 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD54 antigen?

H. Gonzalez

Verified customer

Asked: 2019-04-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-14

Question

Q: can I use citrate plasma as samples in Rat CD54 Picokine® ELISA Kit (Catalog # EK0372)?

Verified Customer

Verified customer

Asked: 2018-08-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD54 causing disruption of its protein structure. CD54 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD54 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-08

Question

Q: how do I analyze ELISA data? I measured CD54 level in plasma.

Verified Customer

Verified customer

Asked: 2014-06-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-06-05

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