PMS2 Antibodies

Mismatch repair endonuclease PMS2 (PMS2)

Part of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2- MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex.

Meeting of the MutL-MutS-heteroduplex ternary complex in existence of RFC and PCNA is enough to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and so generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.

Gene Information

Human
Gene Name:	PMS2
Uniprot:	P54278
Entrez:	5395

Family

Belongs to:
DNA mismatch repair MutL/HexB family

Alternative Names

DNA mismatch repair protein PMS2; EC 3.1; H_DJ0042M02.9; HNPCC4postmeiotic segregation increased (S. cerevisiae) 2; mismatch repair endonuclease PMS2; PMS1 protein homolog 2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMSL2PMS2CL

Molecular Weight

Mass (kDA):
95.797 kDA

Genomic Context

Human
Location:	7p22.1
Sequence:	7; NC_000007.14 (5970925..6009106, complement)

Subcellular Localizations

Nucleus.

Diseases

• Malignant Neoplasms
• Neoplasms
• Hereditary Nonpolyposis Colorectal Cancer
• Colorectal Cancer
• Microsatellite Instability
• Colorectal Neoplasms
• Carcinoma
• Malignant Tumor Of Colon
• Endometrial Polyp
• Turcot Syndrome (disorder)
• Endometrial Carcinoma
• Adenocarcinoma

• Endometrial Neoplasms
• Adenoma
• High-frequency Microsatellite Instability
• Genomic Instability
• Germline Mutation Abnormality
• Brain Neoplasms
• Colonic Neoplasms

Interacting Proteins

• MLH1
• TP73

Pathways

• Mismatch Repair
• Dna Repair
• Methylation
• Pathogenesis
• Dna Replication
• Cell Cycle
• Meiosis
• Localization
• Aging
• Prophase
• Cell Death
• Drug Resistance

• Gene Conversion
• Spermatogenesis
• Cell Proliferation
• Dna Methylation
• Transport
• Hypersensitivity
• Cell Growth

PTMs

• Methylation
• Phosphorylation
• Deamination
• Demethylation

• Cleavage
• Reduction
• Deacetylation
• Acetylation

Research Areas

• Cancer
• Cell Cycle and Replication
• DNA Repair
• Mismatch Repair

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PMS2

Best Uses Of The PMS2 Marker

One of the many benefits of the PMS2 marker is its extensive sensitivity range. The ELISA kits are available upon request. They have been tested on several samples. Picokine(tm) is an extremely sensitive ELISA platform enhances the assay's sensitivity to the level of the picogram. The validation images as well as the procedure are available upon request. It is also possible to use with Supervision the polymer-based secondary antigen that can be used to save up to 30 minutes of IHC. Picoband is built on the insights of Sanbio the distributor of BeNeLux, and technical support.

Boster Bio's research has primary and secondary antibodies

Boster Bio's primary and secondary antibody kits for research using the PMS2 marker include the proteins of interest. They are suitable for immunoblotting and constructed using highly specific, solid antibodies to the PMS2 marker. The antibodies are suitable for multiple applications, including immunohistochemistry, protein analysis, molecular biology, and immunohistochemistry. They are available at competitive prices through a variety of sources.

IHC-optimized polyclonal antibody

Boster Bio is an antibody manufacturer that is specialized in IHC-optimized clonals and picogram-sensitivity ELISA kits. Their product line includes more than 12,000 antibodies that have been tested for WB, IHC, and Flow applications. They also conduct quantitative validations using known quantities Recombinant proteins as well as untransfected cell lines. These antibodies provide high levels of specificity and precision.

The process of optimization is determining the appropriate concentration and diluent needed for IHC-optimized polyclonals. This can help reduce background signals and enhance IHC staining results. These are just a few of the most frequent questions boster bio customers pose. What can IHC-optimized antibodies be used for? Continue reading to learn more. We will address the most common questions regarding IHC-optimized monoclonal antibodies

Quality is the most important factor when it is about IHC optimized polyclonals. Antibodies that are of high quality are more likely to show low cross-reactivity and show the appearance of a narrow banding in background signal. While WB is a good first method for validation monoclonal antibodies work better with native proteins. Polyclonal antibodies on the contrary are more stable to the effects of pH and other environmental variables. These characteristics make polyclonal antibodies more well-suited for IHC/ICC applications.

Boster Bio IHC-optimized Products are available different formats. International distribution can be made by creating custom antibodies or BeNeLux delivery. By choosing the appropriate antibody, you can easily identify the right pathogens with high-quality results. If you are looking for a top-quality product, contact Boster Bio to learn more. Boster Bio is a trusted source of high-affinity primary antibodies.

After selecting the appropriate antibody the tissue is then transferred to the Nitrocellulose membrane and then fixed at 150mA. The membrane is then blocked in 5% Nonfat milk/TBS for 1.5 hours at RT. After blocking the membrane, it is probed with goat anti-mouse HRP secondary antibody at a dilution of 1:5000 in TBS-0.1 percent Tween. The signal is then produced using the Tanon 5200 and Enhanced Cheiluminescent detection kits.