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- Table of Contents
RAD18 Antibodies
AND RAD18 ELISA kits, RAD18 Proteins
Many biological assays use antibodies to detect RAD18, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with RAD18 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop RAD18 antibodies...
Designing a Western blot for RAD18? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the RAD18 Western Blot GuideDesigning an IHC experiment for RAD18? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the RAD18 IHC GuideRAD18 Infographic by Boster Bio
All RAD18 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IHC, WB
$370
Cat # A01622-2
100 μg/vial
Cat # A01622-2
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IP, IF, ICC, WB
$370
Cat # M01623-1
100 μl/vial
Cat # M01623-1
100 μl/vial
RAD18 Overview
Facts about E3 ubiquitin-protein ligase RAD18.
E3 ubiquitin-protein ligase RAD18 (RAD18)
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap- filling of a daughter strand on replication of damaged DNA.
Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Gene Information
| Human | |
|---|---|
| Gene Name: | RAD18 |
| Uniprot: | Q9NS91 |
| Entrez: | 56852 |
Family
| Belongs to: |
|---|
| RAD18 family |
Alternative Names
EC 6.3.2.-; hHR18; hRAD18; postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD18 homolog (S. cerevisiae); RAD18, S. cerevisiae, homolog; RING finger protein 73; RNF73E3 ubiquitin-protein ligase RAD18
Molecular Weight
Mass (kDA):
56.223 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3p25.3 |
| Sequence: | 3; NC_000003.12 (8877075..8969249, complement) |
Subcellular Localizations
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).
Diseases
- Malignant Neoplasms
- Genomic Instability
- Anemia
- Fanconi Anemia
- Neoplasms
- Werner Syndrome
- Xeroderma Pigmentosum
- Genotoxic Stress
- Cytogenetic Abnormality
- Cell Transformation, Neoplastic
- Xeroderma
- Chromosomal Translocation
Interacting Proteins
- HLTF
- MAGEA4
- TAX1BP1
- UBE2A
- UBE2B
Pathways
- Dna Repair
- Dna Replication
- Postreplication Repair
- Translesion Synthesis
- Cell Cycle
- S Phase
- Recombinational Repair
- Localization
- Meiosis
- Hypersensitivity
- Gene Conversion
- Mismatch Repair
- Chromosome Segregation
PTMs
- Ubiquitination
- Phosphorylation
- Sumoylation
- Methylation
Research Areas
- Core ESC-Like Genes
- Stem Cell Markers
- Zinc Finger
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RAD18
bosterbio.com/bosterbio-gene-info-cards/RAD18
References
PMID: 10884424 by Tateishi S., et al. Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.
PMID: 10908344 by Xin H., et al. The human RAD18 gene product interacts with HHR6A and HHR6B.