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Product Info Summary
| SKU: | M01622 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Rad18 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01622
BM4529 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rad18 Rabbit Monoclonal Antibody catalog # M01622. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rad18 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01622)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FFC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rad18
Reactive Species
M01622 is reactive to RAD18 in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
56.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01622 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
FC 1:20
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of RAD18 expression in HeLa cell lysate.
Specific Publications For Anti-Rad18 Rabbit Monoclonal Antibody (M01622)
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4 Customer Q&As for Anti-Rad18 Rabbit Monoclonal Antibody
Question
We are currently using anti-Rad18 Rabbit Monoclonal antibody M01622 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The anti-Rad18 Rabbit Monoclonal antibody (M01622) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-22
Question
Is a blocking peptide available for product anti-Rad18 Rabbit Monoclonal antibody (M01622)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We do provide the blocking peptide for product anti-Rad18 Rabbit Monoclonal antibody (M01622). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-23
Question
Does anti-Rad18 Rabbit Monoclonal antibody M01622 work on dog WB with placenta?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
Our lab technicians have not tested anti-Rad18 Rabbit Monoclonal antibody M01622 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Rad18 Rabbit Monoclonal antibody M01622 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-19
Question
Will M01622 anti-Rad18 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
It shows on the product datasheet, M01622 anti-Rad18 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-04
