Tumor protein 63 Antibodies

Tumor protein 63 (TP63)

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity.

Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes.

Gene Information

Human
Gene Name:	TP63
Uniprot:	Q9H3D4
Entrez:	8626

Family

Belongs to:
p53 family

Alternative Names

amplified in squamous cell carcinoma; Chronic ulcerative stomatitis protein; CUSP; EEC3; EEC3B(p51B); Keratinocyte transcription factor KET; KET; KETRHS; LMS; OFC8; OFC8SHFM4TP73LNBP; p40; p51; p51tumor protein p53-like; p53CP; p63; p63AIS; p73H; p73L; p73LB(p51A); SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; tumor protein p63 deltaN isoform delta; tumor protein p63; Tumor protein p73-like

Molecular Weight

Mass (kDA):
76.785 kDA

Genomic Context

Human
Location:	3q28
Sequence:	3; NC_000003.12 (189596746..189897279)

Tissue Specificity

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.

Subcellular Localizations

Nucleus.

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Malignant Squamous Cell Neoplasm
• Ectodermal Dysplasia
• Cell Transformation, Neoplastic
• Malignant Paraganglionic Neoplasm
• Adenocarcinoma
• Dysplasia
• Neoplasm Metastasis
• Cleft Lip
• Cleft Palate
• Mammary Neoplasms

• Lung Neoplasms
• Carcinogenesis
• Head And Neck Neoplasms
• Ectrodactyly
• Malignant Neoplasm Of Lung
• Hyperplasia
• Malignant Neoplasm Of Breast

Interacting Proteins

• CABLES1
• FUS
• HIPK2
• HNRNPK
• ITCH

• NIPSNAP3A
• PRKCD
• SATB2
• SMAD2
• TP53

• TP53BP2

Pathways

• Cell Cycle
• Cell Proliferation
• Cell Death
• Pathogenesis
• Cell Cycle Arrest
• Cell Growth
• Localization
• Senescence
• Cell Differentiation
• Keratinocyte Differentiation
• Oncogenesis
• Limb Development
• Wound Healing

• Cell Adhesion
• Induction Of Apoptosis
• Cell Division
• Keratinocyte Proliferation
• Rna Interference
• Cell Migration
• Regeneration

PTMs

• Phosphorylation
• Cleavage
• Acetylation
• Sumoylation
• Ubiquitination

• Reduction
• Isgylation
• Deacetylation
• Oxidation
• Methylation

• Ribosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TP63

Best Uses of the TP63 Marker

You might be wondering what the best uses for TP63, picoband, p40, and IHC are. There are a variety of factors to consider. The best way to find out whether an antibody is effective in your experiment is to test it. Let's explore these aspects. We'll go over the various uses of TP63 in this article.

TP63

The TP63 marker is an essential part of the progenitor-cell population which is essential for epithelial development and morphogenesis. Scientists need this protein to carry out these processes. The Boster Bio Anti-p63 antibody interacts with human and bovine cells. Boster Bio Anti-p63 is available in two sizes - one-use vial and an diluted version.

From day 6 to 8, keratinocyte cultures contained the TP63 marker. Immunostaining of the cells revealed that TP63 expression was high. KRT14 is a marker for keratinocytes, was detected using immunostaining on day 6. After eight days of differentiation, 70 percent of the cells that were in the resulting cell cultures were TP63-positive.

Scientists are able to in vitro differentiate human keratinocytes using TP63 markers. This allows them to create unlimited numbers of patient-specific keratinocytes. While in vitro differentiation is essential to obtaining Keratinocytes that are specific to patients the need for new technologies is for lineage-specific differentiation as well as cell expansion for transplantation. In vitro differentiation is a vital step in the creation of patient-specific epidermal stem cells. In vitro epidermal differentiation requires adjustments to the timing of key signaling pathways.

p40 (deltaNp63)

Recent studies suggest that p63 may be useful in clinical settings. However, the limited tissue sample size of tumors can make its use in clinical trials difficult. At present, the function of P63 as a biomarker to aid in differentiation between giant cell tumors has not been fully understood. It is possible to detect p63 in giant cell tumors.

Boster Bio antibody TP63 antigen p40 binds to the p40 protein in bovine mice, human and mouse cells. It also reacts to basal and myoepithelial cells marker. The anti-p40 antibody is compatible with different protocols and substrates, and can be adjusted up or down for optimal performance. This antibody is suitable for immunohistochemistry. It is available in dropper bottles. The antigen can be stored at 2-8oC and azide, or at -20oC sans azide.

Although p40 does not provide a definitive diagnosis for squamous-cell carcinoma, it is a useful marker for differentiation between adenocarcinoma and squamous cells. Positive staining of p40 in adenocarcinoma might be used as a means of subclassification in lung cancer. Although p40 is not an indicator for squamous cells cancer, it could be used to distinguish between cancer types that are subdivided into subtypes.

Picoband

Picoband TP63 Marker is an anti-human antibody. This marker has been utilized in various types of cancers and is a gold standard in multiple diagnostic procedures. It can be used to detect myoepithelial cells that are involved in cancer, and is an effective diagnostic tool for Merkel cell carcinoma. It can also be used to identify the basal cells in the prostate gland and can be used for determining the type of cancer.

The TP63 gene is composed of multiple isoforms. The TAp63 gene has the N-terminal transcriptional domain and is involved in transcription activities. DNp63 isoforms don't contain the transactivation region, and can act as repressors. They can have a positive impact on the transcription of specific genes. The TP63 gene plays a significant role in epithelial growth as well as morphogenesis.

There are two variants of the gene p63: TAp63, or DNp63. Each isoform performs a distinct function in the cell , however they can both bind to the promoter regions of their targets genes. The TAp63 gene is inhibited by the cyclin-dependent-kinase inhibitor 1 (CDKN1A) and HES1 which is a member of the Notch pathway. It also regulates the expression of structural proteins, and helps cells stick together.

IHC

P63 is a protein which is found in a variety of cancer tissues. IHC can reveal the presence of the disease because it is a major component of certain tissue types. For instance, p63 immunohistochemistry can be used to detect basal cells in breast and prostatic glands. It can also be used for diagnosing certain types of tumors, such as squamous cells carcinoma, adenocarcinoma and urothelial. It can also be used to determine if metastasis could be from the primary tumor.

IHC and the TP63 marker have been validated as markers of squamous differentiation. However there are still a lot of questions about the relation between IHC and the TP63 marker. The results of this study are only preliminary. More research is needed to determine the effectiveness of P63-based tumor markers in the clinical setting. This study utilized the TP63 antibody to detect squamous differentiation. There are however some potential advantages of this kind of treatment for cancer patients with the history of lung disease.

To perform IHC and the TP63 marker it is necessary to find anti-TP63 antigens. There is an anti-TP63 antibody available that can be purchased from a commercial source. There are also antibodies that will react to this protein. However the anti-TP63 antibodies must be purchased only from reputable suppliers. A reputable supplier of anti-TP63 antibodies should be able to provide vital information , such as the specificity of the target, and application. It can also be used to test for a broad range of cancers.

ICC

The TP63 marker may be used in ICC to detect metastatic disease and is an excellent biomarker for the detection of abnormal cancer. It is based upon the immunohistochemistry characteristics of tumor samples. When compared to other markers TP63 is positively related to postoperative survival in a small number of cases. It is recommended to use it with caution. This article will provide a brief overview of the most effective uses for the TP63 marker.

In most instances, ICC protein expression is high. It is thought that the expression of these proteins may be related to the prognosis of the patient. This study examined the expression of TP63 and other markers in ICC patients. We also identified clinical characteristics and prognostic factors that were important. These results suggest that TP63 is a significant biomarker in ICC. The marker can also be utilized in other ways.

The p63 marker can be used to diagnose many types of cancer. A tumor that expresses p63 is generally classified as a Urothelial Cancer. A tumor that expresses the p63 gene could be a sarcoma. A bladder tumor with positive p63 results in prostate cancer. This is because the majority of positive p63 bladder tumors are urothelial carcinomas.