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- Table of Contents
TP63 antibody (PB9152) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of A431 cells using anti-p63 antibody (PB9152).
Overlay histogram showing A431 cells stained with PB9152 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p63 Antibody (PB9152, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with PB9152 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p63 Antibody (PB9152, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-p63/TP63 Antibody Picoband®
TP63 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TP63 antibody was positively detected by WB in:
human A431 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TP63 antibody was positively detected by IHC in:
human oesophagus squama cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TP63 antibody was positively detected by ICC/IF in:
A431 cell
TP63 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry
0.5-1μg/ml
Human, -
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-p63/TP63 Antibody Picoband®
View all TP63 Antibodies
View all TP63 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human p63 recombinant protein (Position: S311-E680). Human p63 shares 98% amino acid (aa) sequence identity with both mouse and rat p63.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated Molecular Weight
76.8 kDa
Background of TP63
Tumor protein p63 (TP63), also known as the p63 or TP73L is a protein that in humans is encoded by the TP63 gene. It is mapped to 3q28. TP63 is a member of the p53 family of transcription factors. This gene encodes for two main isoforms by alternative promoters (TAp63 and ΔNp63). TP63 has been mostly restricted to its apoptotic function and more recently as the guardian of oocyte integrity. It has been found that the combined loss of TP63 and p73 results in the failure of cells containing functional p53 to undergo apoptosis in response to DNA damage. TP63 is an essential regulator of stem cell maintenance in stratified epithelial tissues. Tp63 is also critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis.
Cite This Product
Anti-p63/TP63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9152)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rabbit
99%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Dog
98%
High Homology
Mouse
98%
High Homology
Rat
98%
High Homology
Zebrafish
71%
Moderate Homology
Drosophila
9%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TP63 antibody
Immunohistochemistry (IHC) protocol for TP63 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TP63 antibody
Flow Cytometry (FC) protocol for TP63 antibody
Publications
Application:
Species:
Zhong H,Ren Z,Wang X,Miao K,Ni W,Meng Y,Lu L,Wang C,Liu W,Deng CX,Xu RH,Chen G.Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.Int J Biol Sci.2020 Feb 21;16(8):1450-1462.doi:10.7150/ijbs.44414.PMID:32210732;PMCID:PMC7085224.
Species: Human,Mouse
PMID: 32210732
Hu L,Sun Y,Luo J,He X,Ye M,Li G,Zhang Y,Bai J,Zhang D,Chang C.Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression.Oncogene.2020 Feb;39
Species: Human
PMID: 31748715
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Product Promise
We promise Anti-p63/TP63 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9152
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026