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Product Info Summary
| SKU: | M00167-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-p63 TP63 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00167-1
BM4498 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-p63 TP63 Rabbit Monoclonal Antibody catalog # M00167-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p63 TP63 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00167-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FCB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human p63
Reactive Species
M00167-1 is reactive to TP63 in Human, Mouse, Rat
Observed Molecular Weight
77 kDa
Calculated molecular weight
76.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00167-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human RT4 whole cell, human Hacat whole cell, human A431 whole cell
IHC: Human thyroid cancer tissue, Human prostate cancer tissue, Human small cell lung cancer tissue, human breast tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of P63 using anti-P63 antibody (M00167-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P63 antigen affinity purified monoclonal antibody (M00167-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P63 at approximately 77 kDa. The expected band size for P63 is at 63 kDa.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human small cell lung cancer , using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded human breast, using p63 Antibody.
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Immunofluorescent analysis of A431 cells, using p63 Antibody.
Specific Publications For Anti-p63 TP63 Rabbit Monoclonal Antibody (M00167-1)
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5 Customer Q&As for Anti-p63 TP63 Rabbit Monoclonal Antibody
Question
you antibody using your anti-p63 Rabbit Monoclonal antibody for negative regulation of keratinocyte differentiation studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Thanks for your inquiry. This M00167-1 anti-p63 Rabbit Monoclonal antibody is tested on a431 cell lysate. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-09
Question
We have observed staining in rat lymph. Do you have any suggestions? Is anti-p63 Rabbit Monoclonal antibody supposed to stain lymph positively?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
From literature lymph does express TP63. From Uniprot.org, TP63 is expressed in skin of abdomen, keratinocyte skeletal muscle, skeletal muscle, lymph, prostate, placenta, among other tissues. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378
Boster Scientific Support
Answered: 2019-08-14
Question
Our team were satisfied with the WB result of your anti-p63 Rabbit Monoclonal antibody. However we have seen positive staining in placenta nucleus using this antibody. Is that expected? Could you tell me where is TP63 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
From what I have seen in literature, placenta does express TP63. Generally TP63 expresses in nucleus. Regarding which tissues have TP63 expression, here are a few articles citing expression in various tissues:
Keratinocyte, and Skeletal muscle, Pubmed ID: 9799841
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 11477076
Prostate, Pubmed ID: 9662346
Skeletal muscle, Pubmed ID: 9662378
Boster Scientific Support
Answered: 2019-06-26
Question
We have tried in the past anti-p63 Rabbit Monoclonal antibody for IHC on prostate a few years ago. I am using rat, and We are going to use the antibody for ICC next. you antibody examining prostate as well as placenta in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
I viewed the website and datasheets of our anti-p63 Rabbit Monoclonal antibody and I see that M00167-1 has been validated on rat in both IHC and ICC. Thus M00167-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-p63 Rabbit Monoclonal antibody M00167-1 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
The anti-p63 Rabbit Monoclonal antibody (M00167-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-21
