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- Table of Contents
Facts about Cdc42-interacting protein 4.
Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruitment WASL/N- WASP that in turn activates the Arp2/3 complex.
Human | |
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Gene Name: | TRIP10 |
Uniprot: | Q15642 |
Entrez: | 9322 |
Belongs to: |
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FNBP1 family |
cdc42-interacting protein 4; CIP4STOT; HSTP; Protein Felic; Salt tolerant protein; salt tolerator; STPTRIP-10; thyroid hormone receptor interactor 10; thyroid receptor interacting protein 10; Thyroid receptor-interacting protein 10; TR-interacting protein 10
Mass (kDA):
68.352 kDA
Human | |
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Location: | 19p13.3 |
Sequence: | 19; NC_000019.10 (6739680..6751530) |
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection, phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.; [Isoform 5]: Cytoplasm, perinuclear region.
You have come to the right spot if you're looking high-affinity TRIP10 antibodies. Boster sells high-affinity primaries. They've been widely cited and validated in Western Blotting, Immunohistochemistry, and ELISA. Continue reading to learn about the best uses of this TRIP10 antibody. You will also find helpful information.
High-affinity primary antibodies can be used for a variety of applications in biology, biotechnology, and other fields of science. These antibodies are often monoclonal or multiclonal in nature, and are highly specific and cited. Boster Bio offers high-affinity primaries antibodies using the TRIP10 marker. These antibodies have been tested in WB, IHC, and IP.
ECL Chemistry allows researchers to detect the expression of protein using a simple enzyme reaction. The system's main drawback is the inability to capture both enzymatic reactions at the same time and at the same place each time. It cannot also multiplex signals or detect targets with the exact same molecular weight. This limits its usefulness for precise quantification.
The luminol-based substrate that is commonly used in Western Blot applications is the basis of the chemiluminescent technique. Luminescence results from the reaction between HRP-labeled antibodies and a substrate containing the ECL reagent. ECL chemistry allows indirect protein measurement and pictogram detection using imaging instruments.
The ECL Plex Western Blotting System features antibodies that are optimized for quantitative analyses with fluorescent western blotting. The Hybond ECL membrane boasts a pore diameter of 0.45um. This allows for high sensitivity and low background. The ECL Plex Western Blotting System works with proteins of all sizes. These antibodies are highly sensitive and provide good signal-to-noise ratios.
The reporter enzyme forms the basis of the ECL chemical chemiluminescent detection process. The reagents available are luminol-based, acridan-based, or 1,2-dioxetane-based. Luminol, the most popular chemiluminescent reagent, is widely used. It reacts with an enzyme in order to release photons.
The sensitivity of the ECL chemiluminescent detection method is optimized for various applications. The Pierce ECL substrate is suitable for western blotting with many proteins. The Bio Rad Clarity Max ECL substrate improves the system's sensitivities. The half-life of Luminol is approximately one minute. The Bio-Rad Clear Max ECL substrate extends that to 24 hours. This allows for reimaging without loss of signal intensity.
The C-DiGit(r), Blot Scanner eliminates expensive darkroom reagents. C-DiGit is able to capture multiple exposures with its large dynamic range. The iBright Imaging system features a 9.1 MP cooled CCCD camera. It can take more than ten,000,000 exposures to achieve more precise results.
The DAB chromogenic detection system from Boster Bio is a powerful intensification system that significantly enhances the chromogenic signal in immunohistochemistry samples. It uses a catalytic process to create two distinct Linkers: streptavidin-peroxidase conjugate and diaminobenzidine (DAB).
TRIP10 is a rare protein that can be found in many cancer cells. While there is some debate about the exact function of this protein, it is generally accepted to be a member in the human Leukemia virus (Leukemia Virus). Boster Bio's TRIP10 marker provides researchers with high-affinity antibodies for use in Western blotting, immunohistochemistry, and ELISA.
PMID: 9210375 by Aspenstroem P.; A Cdc42 target protein with homology to the non-kinase domain of FER has a potential role in regulating the actin cytoskeleton.
PMID: 12054674 by Wang L., et al. Identification and genetic analysis of human and mouse activated Cdc42 interacting protein-4 isoforms.