C5orf38 (NM_178569) Human Over-expression Lysate

Lysate of C5orf38 over-expression cell lines, WB control

Transient overexpression lysate of chromosome 5 open reading frame 38 (C5orf38)

Product Info Summary

SKU: LS153571
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of chromosome 5 open reading frame 38 (C5orf38)

Product Name

C5orf38 (NM_178569) Human Over-expression Lysate

See all C5orf38 products

SKU/Catalog Number

LS153571

Size

100 µg

Description

Transient overexpression lysate of chromosome 5 open reading frame 38 (C5orf38)

Storage & Handling

Cite This Product

C5orf38 (NM_178569) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS153571)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Components

1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer

1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer

1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for C5orf38 (Source: Uniprot.org, NCBI)

Gene Name

C5orf38

Full Name

Protein CEI

Weight

15.091kDa

Alternative Names

Protein CEI C5orf38 CEI, IRX2NB chromosome 5 open reading frame 38 IRX2 neighbor|coordinated expression to IRX2|coordinated expression to IRXA2 homeobox|coordinated expression to IRXA2 protein

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on C5orf38, check out the C5orf38 Infographic

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In this infographic, you will see the following information for C5orf38: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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