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Product Info Summary
| SKU: | LS027091 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of calcium channel, voltage-dependent, gamma subunit 5 (CACNG5), transcript variant 1 |
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Product info
Product Name
CACNG5 (NM_145811) Human Over-expression Lysate
SKU/Catalog Number
LS027091
Size
100 µg
Description
Transient overexpression lysate of calcium channel, voltage-dependent, gamma subunit 5 (CACNG5), transcript variant 1
Storage & Handling
Cite This Product
CACNG5 (NM_145811) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS027091)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CACNG5 Human Over-expression Lysates NM_145811.
Western blot validation of overexpression lysate (LS027091) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CACNG5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CACNG5 (Source: Uniprot.org, NCBI)
Gene Name
CACNG5
Full Name
Voltage-dependent calcium channel gamma-5 subunit
Weight
30.903kDa
Superfamily
PMP-22/EMP/MP20 family
Alternative Names
calcium channel, voltage-dependent, gamma subunit 5; MGC126656; MGC126682; Neuronal voltage-gated calcium channel gamma-5 subunit; voltage-dependent calcium channel gamma-5 subunit CACNG5 calcium voltage-gated channel auxiliary subunit gamma 5 voltage-dependent calcium channel gamma-5 subunit|TARP gamma-5|calcium channel, voltage-dependent, gamma subunit 5|neuronal voltage-gated calcium channel gamma-5 subunit|transmembrane AMPAR regulatory protein gamma-5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CACNG5, check out the CACNG5 Infographic
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Specific Publications For CACNG5 (NM_145811) Human Over-expression Lysate (LS027091)
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No publications found for LS027091
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