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Product Info Summary
| SKU: | LS124583 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of calcium activated nucleotidase 1 (CANT1), transcript variant 1 |
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Product info
Product Name
CANT1 (NM_138793) Human Over-expression Lysate
See all Calcium Activated Nucleotidase 1/CANT1 products
SKU/Catalog Number
LS124583
Size
100 µg
Description
Transient overexpression lysate of calcium activated nucleotidase 1 (CANT1), transcript variant 1
Storage & Handling
Cite This Product
CANT1 (NM_138793) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS124583)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CANT1 Human Over-expression Lysates NM_001159772.
Western blot validation of overexpression lysate (LS124583) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CANT1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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CANT1 Human Over-expression Lysates NM_001159773.
Western blot validation of overexpression lysate (LS124583) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CANT1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
CANT1 Human Over-expression Lysates NM_138793.
Western blot validation of overexpression lysate (LS124583) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CANT1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for Cant1 (Source: Uniprot.org, NCBI)
Gene Name
Cant1
Full Name
Soluble calcium-activated nucleotidase 1
Weight
45.653kDa
Superfamily
apyrase family
Alternative Names
Apyrase homolog; calcium activated nucleotidase 1; CANT1; DBQD; EC 3.6.1.6; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; SCAN1; SCAN-1Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase; SHAPY; SHAPYmicromelic dwarfism with vertebral and metaphyseal abnormalities and advancedcarpotarsal ossification; soluble Ca-activated nucleotidase, isozyme 1; soluble calcium-activated nucleotidase 1; soluble calcium-activated nucleotidase SCAN-1 Cant1|5830420C20Rik, Apy, Apy1h, D11Bwg0554e, Entpd8, SCA, SCAN-1, Sh, Shapy|calcium activated nucleotidase 1|soluble calcium-activated nucleotidase 1|Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase|apyrase 1, homolog (C. lectularius)|apyrase homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on Cant1, check out the Cant1 Infographic
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In this infographic, you will see the following information for Cant1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CANT1 (NM_138793) Human Over-expression Lysate (LS124583)
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