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Product Info Summary
| SKU: | LS166979 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 1 |
Product info
Product Name
CDC20B (NM_152623) Human Over-expression Lysate
SKU/Catalog Number
LS166979
Size
100 µg
Description
Transient overexpression lysate of cell division cycle 20 homolog B (S. cerevisiae) (CDC20B), transcript variant 1
Storage & Handling
Cite This Product
CDC20B (NM_152623) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS166979)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CDC20B Human Over-expression Lysates NM_001170402.
Western blot validation of overexpression lysate (LS166979) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CDC20B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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CDC20B Human Over-expression Lysates NM_152623.
Western blot validation of overexpression lysate (LS166979) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CDC20B (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CDC20B (Source: Uniprot.org, NCBI)
Gene Name
CDC20B
Full Name
Cell division cycle protein 20 homolog B
Weight
57.335kDa
Superfamily
WD repeat CDC20/Fizzy family
Alternative Names
CDC20 cell division cycle 20 homolog B (S. cerevisiae); CDC20 cell division cycle 20 homolog B; CDC20-like protein; cell division cycle 20 homolog B (S. cerevisiae); cell division cycle protein 20 homolog B; FLJ37927 CDC20B G6VTS76519 cell division cycle 20B cell division cycle protein 20 homolog B|CDC20 cell division cycle 20 homolog B|CDC20-like protein|cell division cycle 20 homolog B
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CDC20B, check out the CDC20B Infographic
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In this infographic, you will see the following information for CDC20B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CDC20B (NM_152623) Human Over-expression Lysate (LS166979)
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No publications found for LS166979
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