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Product Info Summary
| SKU | AR0024 |
|---|---|
| Pack size | 2000mL/pack |
| Form | Dry-blend powder, sufficient to make 2000mL of Citrate Buffer |
| Applications | IHC-P, HIER, PEFF tissues |
Product info
Overview
| Product Name | Citrate Buffer Pack |
|---|---|
| SKU/Catalog Number | AR0024 |
| Pack Size | 2000mL/pack | Form | Dry-blend powder, sufficient to make 2000mL of Citrate Buffer |
| Volume of Final Solution | 2000 mL |
| pH | 6 |
| Buffer Contents | 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O |
| Composition | Sodium citrate.H2O |
| Storage | Store at room temperature. It is stable at room temperature for one year. |
| Application | IHC-P, HIER, PEFF tissues *Our Boster Guarantee covers the use of this product in the above tested applications. |
| Equivalent | Abcam (Product No. ab64214, ab64236) |
| Description | Boster’s Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation. |
| Cite This Product | Citrate Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0024) |
Chemical Information
| CAS Number | Trisodium citrate | 68-04-2 |
|---|---|---|
| Citric acid | 77-92-9 | |
| Molecular Weight | Trisodium citrate | 258.07 |
| Citric acid | 192.123 | |
| Molecular Formula | Trisodium citrate | C6H5Na3O7 |
| Citric acid | C6H8O7 | |
| Chemical Sources | Trisodium citrate | ChEMBL: ChEMBL1355 |
| ChemSpider: 5989 | ||
| PubChem: 6224 | ||
| Citric acid | ChEMBL: ChEMBL1261 | |
| ChemSpider: 305 | ||
| PubChem: 311 |
Usage and Handling
See the IHC protocol for more specific usage instructions.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficient retrieval or avoid tissue destruction or over digestion, appropriate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody, or tissue type after preliminary experimentation by the researcher.
Note: All of Boster's products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use.
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Chemical structure of Sodium Citrate
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Specific Publications For Citrate Buffer Powder (AR0024)
AR0024 has been cited in 35 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Osteopontin mediates murine transfusion-related acute lung injury via stimulation of pulmonary neutrophil accumulation
Qu Y,Dou P,Hu M,Xu J,Xia W,Sun H.circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis.Mol Med Rep.2019 Apr;19(4):3314-3320.doi:10.3892/mmr.2019.9965.Epub 2019 Feb 18.PMID:30816475.
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION: NA
Jia S,Guo P,Lu J,Huang X,Deng L,Jin Y,Zhao L,Fan X.Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice.Drug Des Devel Ther.2021 Jun 21;15:2641-2651.doi:10.2147/DDDT.S292642.PMID:34188444;PMCID:PMC8232843.
Species: Mouse
AR0024 usage in article: APP:IHC, SAMPLE:AIRWAY TISSUE, DILUTION:NA
Su,Y.,Zeng,Z.,Rong,D.,Yang,Y.,Wu,B.,& Cao,Y.(2021).PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma.Oncology Letters,21,289.https://doi.org/10.3892/ol.2021.1255
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:HNSCC TISSUE, DILUTION:NA
Liu,C.,Zhao,C.,Huang,Y.et al.Cell mechanics characteristics of anti-HER2 modified [email protected] and its photothermal treatment of SKOV-3 cells.Appl Nanosci (2021).https://doi.org/10.1007/s13204-021-01674-1
Species: Human
AR0024 usage in article: APP:SYNTHESIS OF NANOMATERIAL, SAMPLE:NA, DILUTION:NA
Baojian Wang,Chunyu Gao,Liguo Zhu et al.Lumbar Instability Induces Ligamentum Flavum Hypertrophy in a Rat Model, 08 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-140228/v1]
Species: Human,Rat
AR0024 usage in article: APP:IHC, SAMPLE:POSTERIOR LUMBAR SPINAL TISSUE, DILUTION:NA
Liu MZ,Chen SM,Xu Y,Kong YG,Deng YQ,Li F,Tao ZZ.Increased Expressions and Roles of CC Chemokine Ligand 21 and CC Chemokine Ligand 25 in Chronic Rhinosinusitis with Nasal Polyps.Int Arch Allergy Immunol.2020;181(3):159-169.doi:10.1159/000504476.Epub 2019 D
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:NPS, DILUTION:NA
Lou X,Fu J,Zhao X,Zhuansun X,Rong C,Sun M,Niu H,Wu L,Zhang Y,An L,Guo L,Wan S,Wang S.MiR-7e-5p downregulation promotes transformation of low-grade follicular lymphoma to aggressive lymphoma by modulating an immunosuppressive stroma through the upregulatio
Species: Human, Mouse
AR0024 usage in article: APP:IHC, SAMPLE:LYMOH NODE TISSUE, DILUTION:NA
The diabetes drug semaglutide reduces infarct size, inflammation, and apoptosis, and normalizes neurogenesis in a rat model of stroke Xiaoyan Yang 1, Peng Feng 2, Xiangjian Zhang 3, Dongfang Li 1, Ruifang Wang 1, Chenhui Ji 4, Guanglai Li 1, Christian H
Overexpression of malignant brain tumor domain containing protein 1 predicts a poor prognosis of prostate cancer Wanhua Wu,1,2,* Shoumin Bai,1,* Dingjun Zhu,1,2,* Kaiwen Li,1,2 Wen Dong,1,2 Wang He,1,2 Shengmeng Peng,1,2 Yiming Lai,1,2 Qiong Wang,1,2 Zh
Androgen deprivation therapy has no effect on Pim-1 expression in a mouse model of prostate cancer
The Prognostic Role of SIRT1-Autophagy Axis in Gastric Cancer
Involvement of Activated Cdc42 Kinase1 in Colitis and Colorectal Neoplasms
High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium
Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer
Expression of hypoxia-inducible factor 3? in hepatocellular carcinoma and its association with other hypoxia-inducible factors
Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing
Liang-Ge-San, a classic traditional Chinese medicine formula, protects against lipopolysaccharide-induced inflammation through cholinergic anti-inflammatory pathway
Expression of secreted phospholipase A2-Group IIA correlates with prognosis of gastric adenocarcinoma
Role of nitric oxide synthase in the development of bone cancer pain and effect of L-NMMA
Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma
Reductions in calcitonin gene-related peptide may be associated with the impairment of the contralateral testis in unilateral cryptorchidism
Effect of anterior cervical discectomy and fusion on adjacent segments in rabbits
Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-?B in Acute Transection Spinal Cord Injury in Rats
Jia L, Li F, Shao M, Zhang W, Zhang C, Zhao X, Luan H, Qi Y, Zhang P, Liang L, Jia X, Zhang K, Lu Y, Yang Z, Zhu X, Zhang Q, Du J, Wang W Oncol Lett. 2018
Ju X, Shen R, Huang P, Zhai J, Qian X, Wang Q, Chen M,. Oncotarget. 2017 Oct 26;8(59):99372-99381. doi: 10.18632/oncotarget.22079. eCollection 2017 Nov 21. Predictive relevance of PD-L1 expression with pre-existing TILs in gastric cancer
Ju T, Jin H, Ying R, Xie Q, Zhou C, Gao D. Mol Med Rep. 2017 Sep;16(3):3194-3200. doi: 10.3892/mmr.2017.6986. Epub 2017 Jul 14. Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells
Liu L, Lai YJ, Zhao LG, Chen GJ. Exp Ther Med. 2017 Dec;14(6):5751-5756. doi: 10.3892/etm.2017.5289. Epub 2017 Oct 11. Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice
Jiang Y, Zhao Y, Chen S, Chen L, Li C, Zhou X. Reprod Biol Endocrinol. 2018 Mar 20;16(1):25. doi: 10.1186/s12958-018-0348-8. Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary
Yang X, Zhang D, Chong T, Li Y, Wang Z, Zhang P. Oncol Lett. 2018 Apr;15(4):4229-4234. doi: 10.3892/ol.2018.7871. Epub 2018 Jan 26. Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma
Liang K, Liu Y, Eer D, Liu J, Yang F, Hu K. Med Sci Monit. 2018 Jan 21;24:405-411. High CXC Chemokine Ligand 16 (CXCL16) Expression Promotes Proliferation and Metastasis of Lung Cancer via Regulating the NF-κB Pathway.
Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.
Li K, Wang J, Yin X, Zhai X, Li Z. Int J Mol Sci. 2013 Mar 15;14(3):5998-6011. Doi: 10.3390/Ijms14035998. Alteration Of Podocyte Protein Expression And Localization In The Early Stage Of Various Hemodynamic Conditions.
Yuan X, Wang W, Wang J, Yin X, Zhai X, Wang L, Li K, Li Z. Plos One. 2014 Apr 4;9(4):E94212. Doi: 10.1371/Journal.Pone.0094212. Ecollection 2014. Down-Regulation Of Integrin ??1 And Focal Adhesion Kinase In Renal Glomeruli Under Various Hemodynami...
Dong Xm, Zhu Ym, Cai H, Lv C, Gao Yr, Yu Z, Xue F. Vet Microbiol. 2012 Jul 6;158(1-2):199-204. Doi: 10.1016/J.Vetmic.2012.02.012. Epub 2012 Feb 17. Studies On The Pathogenesis Of A Chinese Strain Of Bovine Parainfluenza Virus Type 3 Infection In B...
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Customer Q&As
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2 Customer Q&As for Citrate Buffer Powder
Question
What is the weight AR0024 supplied in grams?
Verified customer
Asked: 2020-11-30
Answer
For the Citrate Buffer Powder (AR0024), the weight of the powder supplied is 23g. Buffer Contents: 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
Boster Scientific Support
Answered: 2020-11-30
Question
Can AR0024 be used for the antigen retrieval in the PA2079 protocol as well?
Verified customer
Asked: 2019-04-18
Answer
Yes, Citrate Buffer Powder AR0024 can be used for the antigen retrieval in the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079) protocol.
Boster Scientific Support
Answered: 2019-04-19
