Citrate Buffer Powder

antibody

Boster’s Citrate Buffer Pack is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation. Cited in 74 publication(s).

Product Info Summary

SKU: AR0024
Size: 1 pack

Overview

Product Name Citrate Buffer Pack
SKU/Catalog Number AR0024
Pack Size 2000mL/pack
Form Dry-blend powder, sufficient to make 2000mL of Citrate Buffer
Volume of Final Solution 2000 mL
pH6
Buffer Contents 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
Composition Sodium citrate.H2O
Storage Store at room temperature. It is stable at room temperature for one year.
Application IHC-P, HIER, PEFF tissues
*Our Boster Guarantee covers the use of this product in the above tested applications.
Equivalent Abcam (Product No. ab64214, ab64236)
Description Boster’s Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.
Cite This Product Citrate Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0024)

Chemical Information

CAS NumberTrisodium citrate68-04-2
Citric acid77-92-9
Molecular WeightTrisodium citrate258.07
Citric acid192.123
Molecular FormulaTrisodium citrateC6H5Na3O7
Citric acidC6H8O7
Chemical SourcesTrisodium citrateChEMBL: ChEMBL1355
ChemSpider: 5989
PubChem: 6224
Citric acidChEMBL: ChEMBL1261
ChemSpider: 305
PubChem: 311

Usage and Handling

See the IHC protocol for more specific usage instructions.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficient retrieval or avoid tissue destruction or over digestion, appropriate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody, or tissue type after preliminary experimentation by the researcher.
Note: All of Boster's products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use.

Validation Images & Assay Conditions

AR0024 has been cited in 74 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

SNX-2112 Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Non-Small Cell Lung Cancer by Downregulating Epithelial-Mesenchymal Transition via the Wnt/β-Catenin Signaling Pathway

Establishing a nonlethal and efficient mouse model of male gonadotoxicity by intraperitoneal busulfan injection

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer

Expression and prognostic value of E2F3 transcription factor in non‑small cell lung cancer

PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma

Flotillin‑2 predicts poor prognosis and promotes tumor invasion in intrahepatic cholangiocarcinoma

Overexpression of malignant brain tumor domain containing protein 1 predicts a poor prognosis of prostate cancer

High levels of glioma tumor suppressor candidate region gene 1 predicts a poor prognosis for prostate cancer

IL‑8 is upregulated in cervical cancer tissues and is associated with the proliferation and migration of HeLa cervical cancer cells

Androgen deprivation therapy has no effect on Pim-1 expression in a mouse model of prostate cancer

Expression of secreted phospholipase A2-Group IIA correlates with prognosis of gastric adenocarcinoma

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis

Effects of mild induced hypothermia on hippocampal connexin 43 and glutamate transporter 1 expression following traumatic brain injury in rats

Detection of mitochondrial coupling factor 6 in placental tissues from preeclamptic pregnancies and its influence on biological behavior of trophoblast cells

Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice

The Role and Mechanism of S1PR5 in Colon Cancer

Inhibition of STAT3 by RNA interference suppresses angiogenesis in colorectal carcinoma

The effect of down-regulation of Smad3 by RNAi on hepatic stellate cells and a carbon tetrachloride-induced rat model of hepatic fibrosis

Cyclopamine Suppresses Human Esophageal Carcinoma Cell Growth by Inhibiting Glioma-Associated Oncogene Protein-1, a Marker of Human Esophageal Carcinoma Progression

Rho/ROCK Pathway Regulates Migration and Invasion of Esophageal Squamous Cell Carcinoma by Regulating Caveolin-1

High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium

LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis

Expression of early growth responsive gene-1 in the visual cortex of monocular form deprivation amblyopic kittens

The Prognostic Role of SIRT1-Autophagy Axis in Gastric Cancer

Effects of folic acid on oligozoospermia with MTHFR polymorphisms in term of seminal parameters, DNA fragmentation, and live birth rate: a double‐blind, randomized, placebo‐controlled trial

Phosphorylated mixed lineage kinase domain‐like protein in human seminal plasma: A potential novel biomarker of spermatogenic function

Urine-Derived Stem Cells Facilitate Endogenous Spermatogenesis Restoration of Busulfan-Induced Nonobstructive Azoospermic Mice by Paracrine Exosomes

Luteolin ameliorates inflammation and Th1/Th2 imbalance via regulating the TLR4/NF-κB pathway in allergic rhinitis rats

Transcriptome profiling reveals morphogenesis-related candidate genes and pathways in the chick embryonic small intestine

Increasing of FKBP9 can predict poor prognosis in patients with prostate cancer

The diabetes drug semaglutide reduces infarct size, inflammation, and apoptosis, and normalizes neurogenesis in a rat model of stroke

Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro

Activation of p38 Mitogen-activated Protein Kinase in Rat Periapical Lesions

Effects of beta-cypermethrin on male rat reproductive system

A novel carbazole-based hydrogen-sulfide donor suppresses seizures and upregulates ATP-sensitive potassium channels

p63 Expression and its Relation to Epithelial Cells Proliferation in Dentigerous Cyst, Odontogenic Keratocyst, and Ameloblastoma

Expression and prognostic value of Ars2 in hepatocellular carcinoma

Ars2 is overexpressed in human cholangiocarcinomas and its depletion increases PTEN and PDCD4 by decreasing microRNA‐21

Osteopontin mediates murine transfusion-related acute lung injury via stimulation of pulmonary neutrophil accumulation

Qu Y,Dou P,Hu M,Xu J,Xia W,Sun H.circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis.Mol Med Rep.2019 Apr;19(4):3314-3320.doi:10.3892/mmr.2019.9965.Epub 2019 Feb 18.PMID:30816475.
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION: NA

Jia S,Guo P,Lu J,Huang X,Deng L,Jin Y,Zhao L,Fan X.Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice.Drug Des Devel Ther.2021 Jun 21;15:2641-2651.doi:10.2147/DDDT.S292642.PMID:34188444;PMCID:PMC8232843.
Species: Mouse
AR0024 usage in article: APP:IHC, SAMPLE:AIRWAY TISSUE, DILUTION:NA

Su,Y.,Zeng,Z.,Rong,D.,Yang,Y.,Wu,B.,& Cao,Y.(2021).PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma.Oncology Letters,21,289.https://doi.org/10.3892/ol.2021.1255
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:HNSCC TISSUE, DILUTION:NA

Liu,C.,Zhao,C.,Huang,Y.et al.Cell mechanics characteristics of anti-HER2 modified [email protected] and its photothermal treatment of SKOV-3 cells.Appl Nanosci (2021).https://doi.org/10.1007/s13204-021-01674-1
Species: Human
AR0024 usage in article: APP:SYNTHESIS OF NANOMATERIAL, SAMPLE:NA, DILUTION:NA

Baojian Wang,Chunyu Gao,Liguo Zhu et al.Lumbar Instability Induces Ligamentum Flavum Hypertrophy in a Rat Model, 08 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-140228/v1]
Species: Human,Rat
AR0024 usage in article: APP:IHC, SAMPLE:POSTERIOR LUMBAR SPINAL TISSUE, DILUTION:NA

Liu MZ,Chen SM,Xu Y,Kong YG,Deng YQ,Li F,Tao ZZ.Increased Expressions and Roles of CC Chemokine Ligand 21 and CC Chemokine Ligand 25 in Chronic Rhinosinusitis with Nasal Polyps.Int Arch Allergy Immunol.2020;181(3):159-169.doi:10.1159/000504476.Epub 2019 D
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:NPS, DILUTION:NA

Lou X,Fu J,Zhao X,Zhuansun X,Rong C,Sun M,Niu H,Wu L,Zhang Y,An L,Guo L,Wan S,Wang S.MiR-7e-5p downregulation promotes transformation of low-grade follicular lymphoma to aggressive lymphoma by modulating an immunosuppressive stroma through the upregulatio
Species: Human, Mouse
AR0024 usage in article: APP:IHC, SAMPLE:LYMOH NODE TISSUE, DILUTION:NA

The diabetes drug semaglutide reduces infarct size, inflammation, and apoptosis, and normalizes neurogenesis in a rat model of stroke Xiaoyan Yang 1, Peng Feng 2, Xiangjian Zhang 3, Dongfang Li 1, Ruifang Wang 1, Chenhui Ji 4, Guanglai Li 1, Christian H

Overexpression of malignant brain tumor domain containing protein 1 predicts a poor prognosis of prostate cancer Wanhua Wu,1,2,* Shoumin Bai,1,* Dingjun Zhu,1,2,* Kaiwen Li,1,2 Wen Dong,1,2 Wang He,1,2 Shengmeng Peng,1,2 Yiming Lai,1,2 Qiong Wang,1,2 Zh

Androgen deprivation therapy has no effect on Pim-1 expression in a mouse model of prostate cancer

The Prognostic Role of SIRT1-Autophagy Axis in Gastric Cancer

Involvement of Activated Cdc42 Kinase1 in Colitis and Colorectal Neoplasms

High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer

Expression of hypoxia-inducible factor 3? in hepatocellular carcinoma and its association with other hypoxia-inducible factors

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

Liang-Ge-San, a classic traditional Chinese medicine formula, protects against lipopolysaccharide-induced inflammation through cholinergic anti-inflammatory pathway

Expression of secreted phospholipase A2-Group IIA correlates with prognosis of gastric adenocarcinoma

Role of nitric oxide synthase in the development of bone cancer pain and effect of L-NMMA

Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma

Reductions in calcitonin gene-related peptide may be associated with the impairment of the contralateral testis in unilateral cryptorchidism

Effect of anterior cervical discectomy and fusion on adjacent segments in rabbits

Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-?B in Acute Transection Spinal Cord Injury in Rats

Jia L, Li F, Shao M, Zhang W, Zhang C, Zhao X, Luan H, Qi Y, Zhang P, Liang L, Jia X, Zhang K, Lu Y, Yang Z, Zhu X, Zhang Q, Du J, Wang W Oncol Lett. 2018

Ju X, Shen R, Huang P, Zhai J, Qian X, Wang Q, Chen M,. Oncotarget. 2017 Oct 26;8(59):99372-99381. doi: 10.18632/oncotarget.22079. eCollection 2017 Nov 21. Predictive relevance of PD-L1 expression with pre-existing TILs in gastric cancer

Ju T, Jin H, Ying R, Xie Q, Zhou C, Gao D. Mol Med Rep. 2017 Sep;16(3):3194-3200. doi: 10.3892/mmr.2017.6986. Epub 2017 Jul 14. Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells

Liu L, Lai YJ, Zhao LG, Chen GJ. Exp Ther Med. 2017 Dec;14(6):5751-5756. doi: 10.3892/etm.2017.5289. Epub 2017 Oct 11. Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice

Jiang Y, Zhao Y, Chen S, Chen L, Li C, Zhou X. Reprod Biol Endocrinol. 2018 Mar 20;16(1):25. doi: 10.1186/s12958-018-0348-8. Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary

Yang X, Zhang D, Chong T, Li Y, Wang Z, Zhang P. Oncol Lett. 2018 Apr;15(4):4229-4234. doi: 10.3892/ol.2018.7871. Epub 2018 Jan 26. Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma

Liang K, Liu Y, Eer D, Liu J, Yang F, Hu K. Med Sci Monit. 2018 Jan 21;24:405-411. High CXC Chemokine Ligand 16 (CXCL16) Expression Promotes Proliferation and Metastasis of Lung Cancer via Regulating the NF-κB Pathway.

Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.

Wang J, Ren Y, Guo X, Cheng H, Ye Y, Qi J, Yang C, You H. Mol Med Rep. 2015 May;11(5):3585-92. Doi: 10.3892/Mmr.2015.3164. Epub 2015 Jan 8. Alterations In Enhancer Of Zeste Homolog 2, Matrix Metalloproteinase-2 And Tissue Inhibitor Of Metalloprote...

Li K, Wang J, Yin X, Zhai X, Li Z. Int J Mol Sci. 2013 Mar 15;14(3):5998-6011. Doi: 10.3390/Ijms14035998. Alteration Of Podocyte Protein Expression And Localization In The Early Stage Of Various Hemodynamic Conditions.

Yuan X, Wang W, Wang J, Yin X, Zhai X, Wang L, Li K, Li Z. Plos One. 2014 Apr 4;9(4):E94212. Doi: 10.1371/Journal.Pone.0094212. Ecollection 2014. Down-Regulation Of Integrin ??1 And Focal Adhesion Kinase In Renal Glomeruli Under Various Hemodynami...

Dong Xm, Zhu Ym, Cai H, Lv C, Gao Yr, Yu Z, Xue F. Vet Microbiol. 2012 Jul 6;158(1-2):199-204. Doi: 10.1016/J.Vetmic.2012.02.012. Epub 2012 Feb 17. Studies On The Pathogenesis Of A Chinese Strain Of Bovine Parainfluenza Virus Type 3 Infection In B...

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2 Customer Q&As for Citrate Buffer Powder

Question

What is the weight AR0024 supplied in grams?

Verified customer

Asked: 2020-11-30

Answer

For the Citrate Buffer Powder (AR0024), the weight of the powder supplied is 23g. Buffer Contents: 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O

Boster Scientific Support

Answered: 2020-11-30

Question

Can AR0024 be used for the antigen retrieval in the PA2079 protocol as well?

Verified customer

Asked: 2019-04-18

Answer

Yes, Citrate Buffer Powder AR0024 can be used for the antigen retrieval in the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079) protocol.

Boster Scientific Support

Answered: 2019-04-19

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