Citrate Buffer Powder

Boster’s Citrate Buffer Pack is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation. Cited in 35 publication(s).

Product Info Summary

SKU AR0024
Pack size 2000mL/pack
Form Dry-blend powder, sufficient to make 2000mL of Citrate Buffer
Applications IHC-P, HIER, PEFF tissues


Product Name Citrate Buffer Pack
SKU/Catalog Number AR0024
Pack Size 2000mL/pack
Form Dry-blend powder, sufficient to make 2000mL of Citrate Buffer
Volume of Final Solution 2000 mL
Buffer Contents 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O
Composition Sodium citrate.H2O
Storage Store at room temperature. It is stable at room temperature for one year.
Application IHC-P, HIER, PEFF tissues
*Our Boster Guarantee covers the use of this product in the above tested applications.
Equivalent Abcam (Product No. ab64214, ab64236)
Description Boster’s Citrate buffer solution is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.
Cite This Product Citrate Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0024)

Chemical Information

CAS NumberTrisodium citrate68-04-2
Citric acid77-92-9
Molecular WeightTrisodium citrate258.07
Citric acid192.123
Molecular FormulaTrisodium citrateC6H5Na3O7
Citric acidC6H8O7
Chemical SourcesTrisodium citrateChEMBL: ChEMBL1355
ChemSpider: 5989
PubChem: 6224
Citric acidChEMBL: ChEMBL1261
ChemSpider: 305
PubChem: 311

Usage and Handling

See the IHC protocol for more specific usage instructions.
Certain antigens may utilize a combination of retrieval methods. Antigen retrieval may not be required for frozen cross section as cross links are formed during formalin fixation.
Advice: In order to achieve sufficient retrieval or avoid tissue destruction or over digestion, appropriate temperature, pH, buffer concentration, incubation times, and other conditions should be carefully selected for each particular antigen, antibody, or tissue type after preliminary experimentation by the researcher.
Note: All of Boster's products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use.

Product Images

AR0024 has been cited in 35 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Osteopontin mediates murine transfusion-related acute lung injury via stimulation of pulmonary neutrophil accumulation

Qu Y,Dou P,Hu M,Xu J,Xia W,Sun H.circRNA‑CER mediates malignant progression of breast cancer through targeting the miR‑136/MMP13 axis.Mol Med Rep.2019 Apr;19(4):3314-3320.doi:10.3892/mmr.2019.9965.Epub 2019 Feb 18.PMID:30816475.
Species: Human

Jia S,Guo P,Lu J,Huang X,Deng L,Jin Y,Zhao L,Fan X.Curcumol Ameliorates Lung Inflammation and Airway Remodeling via Inhibiting the Abnormal Activation of the Wnt/β-Catenin Pathway in Chronic Asthmatic Mice.Drug Des Devel Ther.2021 Jun 21;15:2641-2651.doi:10.2147/DDDT.S292642.PMID:34188444;PMCID:PMC8232843.
Species: Mouse

Su,Y.,Zeng,Z.,Rong,D.,Yang,Y.,Wu,B.,& Cao,Y.(2021).PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV‑negative head and neck squamous cell carcinoma.Oncology Letters,21,289.
Species: Human

Liu,C.,Zhao,C.,Huang, al.Cell mechanics characteristics of anti-HER2 modified [email protected] and its photothermal treatment of SKOV-3 cells.Appl Nanosci (2021).
Species: Human

Baojian Wang,Chunyu Gao,Liguo Zhu et al.Lumbar Instability Induces Ligamentum Flavum Hypertrophy in a Rat Model, 08 January 2021, PREPRINT (Version 1) available at Research Square []
Species: Human,Rat

Liu MZ,Chen SM,Xu Y,Kong YG,Deng YQ,Li F,Tao ZZ.Increased Expressions and Roles of CC Chemokine Ligand 21 and CC Chemokine Ligand 25 in Chronic Rhinosinusitis with Nasal Polyps.Int Arch Allergy Immunol.2020;181(3):159-169.doi:10.1159/000504476.Epub 2019 D
Species: Human
AR0024 usage in article: APP:IHC, SAMPLE:NPS, DILUTION:NA

Lou X,Fu J,Zhao X,Zhuansun X,Rong C,Sun M,Niu H,Wu L,Zhang Y,An L,Guo L,Wan S,Wang S.MiR-7e-5p downregulation promotes transformation of low-grade follicular lymphoma to aggressive lymphoma by modulating an immunosuppressive stroma through the upregulatio
Species: Human, Mouse

The diabetes drug semaglutide reduces infarct size, inflammation, and apoptosis, and normalizes neurogenesis in a rat model of stroke Xiaoyan Yang 1, Peng Feng 2, Xiangjian Zhang 3, Dongfang Li 1, Ruifang Wang 1, Chenhui Ji 4, Guanglai Li 1, Christian H

Overexpression of malignant brain tumor domain containing protein 1 predicts a poor prognosis of prostate cancer Wanhua Wu,1,2,* Shoumin Bai,1,* Dingjun Zhu,1,2,* Kaiwen Li,1,2 Wen Dong,1,2 Wang He,1,2 Shengmeng Peng,1,2 Yiming Lai,1,2 Qiong Wang,1,2 Zh

Androgen deprivation therapy has no effect on Pim-1 expression in a mouse model of prostate cancer

The Prognostic Role of SIRT1-Autophagy Axis in Gastric Cancer

Involvement of Activated Cdc42 Kinase1 in Colitis and Colorectal Neoplasms

High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium

Decreased LIPF expression is correlated with DGKA and predicts poor outcome of gastric cancer

Expression of hypoxia-inducible factor 3? in hepatocellular carcinoma and its association with other hypoxia-inducible factors

Preclinical pharmacodynamic evaluation of antibiotic nitroxoline for anticancer drug repurposing

Liang-Ge-San, a classic traditional Chinese medicine formula, protects against lipopolysaccharide-induced inflammation through cholinergic anti-inflammatory pathway

Expression of secreted phospholipase A2-Group IIA correlates with prognosis of gastric adenocarcinoma

Role of nitric oxide synthase in the development of bone cancer pain and effect of L-NMMA

Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma

Reductions in calcitonin gene-related peptide may be associated with the impairment of the contralateral testis in unilateral cryptorchidism

Effect of anterior cervical discectomy and fusion on adjacent segments in rabbits

Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-?B in Acute Transection Spinal Cord Injury in Rats

Jia L, Li F, Shao M, Zhang W, Zhang C, Zhao X, Luan H, Qi Y, Zhang P, Liang L, Jia X, Zhang K, Lu Y, Yang Z, Zhu X, Zhang Q, Du J, Wang W Oncol Lett. 2018

Ju X, Shen R, Huang P, Zhai J, Qian X, Wang Q, Chen M,. Oncotarget. 2017 Oct 26;8(59):99372-99381. doi: 10.18632/oncotarget.22079. eCollection 2017 Nov 21. Predictive relevance of PD-L1 expression with pre-existing TILs in gastric cancer

Ju T, Jin H, Ying R, Xie Q, Zhou C, Gao D. Mol Med Rep. 2017 Sep;16(3):3194-3200. doi: 10.3892/mmr.2017.6986. Epub 2017 Jul 14. Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells

Liu L, Lai YJ, Zhao LG, Chen GJ. Exp Ther Med. 2017 Dec;14(6):5751-5756. doi: 10.3892/etm.2017.5289. Epub 2017 Oct 11. Increased expression of Myc-interacting zinc finger protein 1 in APP/PS1 mice

Jiang Y, Zhao Y, Chen S, Chen L, Li C, Zhou X. Reprod Biol Endocrinol. 2018 Mar 20;16(1):25. doi: 10.1186/s12958-018-0348-8. Regulation by FSH of the dynamic expression of retinol-binding protein 4 in the mouse ovary

Yang X, Zhang D, Chong T, Li Y, Wang Z, Zhang P. Oncol Lett. 2018 Apr;15(4):4229-4234. doi: 10.3892/ol.2018.7871. Epub 2018 Jan 26. Expression of CK19, CD105 and CD146 are associated with early metastasis in patients with renal cell carcinoma

Liang K, Liu Y, Eer D, Liu J, Yang F, Hu K. Med Sci Monit. 2018 Jan 21;24:405-411. High CXC Chemokine Ligand 16 (CXCL16) Expression Promotes Proliferation and Metastasis of Lung Cancer via Regulating the NF-κB Pathway.

Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.

Li K, Wang J, Yin X, Zhai X, Li Z. Int J Mol Sci. 2013 Mar 15;14(3):5998-6011. Doi: 10.3390/Ijms14035998. Alteration Of Podocyte Protein Expression And Localization In The Early Stage Of Various Hemodynamic Conditions.

Yuan X, Wang W, Wang J, Yin X, Zhai X, Wang L, Li K, Li Z. Plos One. 2014 Apr 4;9(4):E94212. Doi: 10.1371/Journal.Pone.0094212. Ecollection 2014. Down-Regulation Of Integrin ??1 And Focal Adhesion Kinase In Renal Glomeruli Under Various Hemodynami...

Dong Xm, Zhu Ym, Cai H, Lv C, Gao Yr, Yu Z, Xue F. Vet Microbiol. 2012 Jul 6;158(1-2):199-204. Doi: 10.1016/J.Vetmic.2012.02.012. Epub 2012 Feb 17. Studies On The Pathogenesis Of A Chinese Strain Of Bovine Parainfluenza Virus Type 3 Infection In B...

Have you used Citrate Buffer Powder?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Citrate Buffer Powder

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

2 Customer Q&As for Citrate Buffer Powder


What is the weight AR0024 supplied in grams?

Verified customer

Asked: 2020-11-30


For the Citrate Buffer Powder (AR0024), the weight of the powder supplied is 23g. Buffer Contents: 3g C6H8O7.H2, 20g C6H5Na3O7.2H2O

Boster Scientific Support

Answered: 2020-11-30


Can AR0024 be used for the antigen retrieval in the PA2079 protocol as well?

Verified customer

Asked: 2019-04-18


Yes, Citrate Buffer Powder AR0024 can be used for the antigen retrieval in the Anti-VE-Cadherin-5 CDH5-Antibody (PA2079) protocol.

Boster Scientific Support

Answered: 2019-04-19


Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product



Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.