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Product Info Summary
| SKU: | LS009337 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of CCR4-NOT transcription complex, subunit 8 (CNOT8) |
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Product info
Product Name
CNOT8 (NM_004779) Human Over-expression Lysate
SKU/Catalog Number
LS009337
Size
100 µg
Description
Transient overexpression lysate of CCR4-NOT transcription complex, subunit 8 (CNOT8)
Storage & Handling
Cite This Product
CNOT8 (NM_004779) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009337)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CNOT8 Human Over-expression Lysates NM_004779.
Western blot validation of overexpression lysate (LS009337) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CNOT8 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CNOT8 (Source: Uniprot.org, NCBI)
Gene Name
CNOT8
Full Name
CCR4-NOT transcription complex subunit 8
Weight
33.54kDa
Superfamily
CAF1 family
Alternative Names
CAF1; CAF2; CALIFCAF1-like protein; CALIFp; CCR4-NOT transcription complex subunit 8; CCR4-NOT transcription complex, subunit 8; hCAF1; PGK promoter directed over production; POP2CCR4-associated factor 8 CNOT8 CAF1, CALIF, Caf1b, POP2, hCAF1 CCR4-NOT transcription complex subunit 8 CCR4-NOT transcription complex subunit 8|CAF1-like protein|CAF2|CALIFp|CCR4-associated factor 8|PGK promoter directed over production
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CNOT8, check out the CNOT8 Infographic
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In this infographic, you will see the following information for CNOT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CNOT8 (NM_004779) Human Over-expression Lysate (LS009337)
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