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Product Info Summary
| SKU: | LS025839 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of component of oligomeric golgi complex 4 (COG4) |
Product info
Product Name
COG4 (NM_015386) Human Over-expression Lysate
SKU/Catalog Number
LS025839
Size
100 µg
Description
Transient overexpression lysate of component of oligomeric golgi complex 4 (COG4)
Storage & Handling
Cite This Product
COG4 (NM_015386) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS025839)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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COG4 Human Over-expression Lysates NM_015386.
Western blot validation of overexpression lysate (LS025839) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with COG4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for COG4 (Source: Uniprot.org, NCBI)
Gene Name
COG4
Full Name
Conserved oligomeric Golgi complex subunit 4
Weight
89.083kDa
Superfamily
COG4 family
Alternative Names
CDG2J; COD1complexed with Dor1p; COG complex subunit 4; component of oligomeric golgi complex 4DKFZp586E1519; conserved oligomeric Golgi complex protein 4; conserved oligomeric Golgi complex subunit 4; DKFZP586E1519 COG4 CDG2J, COD1, SWILS component of oligomeric golgi complex 4 conserved oligomeric Golgi complex subunit 4|COG complex subunit 4|complexed with Dor1p|conserved oligomeric Golgi complex protein 4
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on COG4, check out the COG4 Infographic
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In this infographic, you will see the following information for COG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For COG4 (NM_015386) Human Over-expression Lysate (LS025839)
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No publications found for LS025839
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