Product Info Summary
SKU: | LS008292 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I |
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Product info
Product Name
COLQ (NM_005677) Human Over-expression Lysate
SKU/Catalog Number
LS008292
Size
100 µg
Description
Transient overexpression lysate of collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I
Storage & Handling
Cite This Product
COLQ (NM_005677) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008292)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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COLQ Human Over-expression Lysates NM_005677.
Western blot validation of overexpression lysate (LS008292) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with COLQ (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
COLQ Human Over-expression Lysates NM_080538.
Western blot validation of overexpression lysate (LS008292) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with COLQ (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
COLQ Human Over-expression Lysates NM_080539.
Western blot validation of overexpression lysate (LS008292) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with COLQ (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for COLQ (Source: Uniprot.org, NCBI)
Gene Name
COLQ
Full Name
Acetylcholinesterase collagenic tail peptide
Weight
47.766kDa
Superfamily
COLQ family
Alternative Names
acetylcholinesterase collagenic tail peptide; AChE Q subunit; collagenic tail of endplate acetylcholinesterase; collagen-like tail subunit (single strand of homotrimer) of asymmetricacetylcholinesterase; EADAcetylcholinesterase-associated collagen; FLJ55041; single strand of homotrimeric collagen-like tail subunit of asymmetricacetylcholinesterase COLQ CMS5, EAD collagen like tail subunit of asymmetric acetylcholinesterase acetylcholinesterase collagenic tail peptide|AChE Q subunit|acetylcholinesterase-associated collagen|collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase|collagenic tail of endplate acetylcholinesterase|single strand of homotrimeric collagen-like tail subunit of asymmetric acetylcholinesterase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on COLQ, check out the COLQ Infographic
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In this infographic, you will see the following information for COLQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For COLQ (NM_005677) Human Over-expression Lysate (LS008292)
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