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Product Info Summary
| SKU: | LS001413 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of crystallin, beta A4 (CRYBA4) |
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Product info
Product Name
CRYBA4 (NM_001886) Human Over-expression Lysate
SKU/Catalog Number
LS001413
Size
100 µg
Description
Transient overexpression lysate of crystallin, beta A4 (CRYBA4)
Storage & Handling
Cite This Product
CRYBA4 (NM_001886) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001413)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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CRYBA4 Human Over-expression Lysates NM_001886.
Western blot validation of overexpression lysate (LS001413) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with CRYBA4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for CRYBA4 (Source: Uniprot.org, NCBI)
Gene Name
CRYBA4
Full Name
Beta-crystallin A4
Weight
22.374kDa
Superfamily
beta/gamma-crystallin family
Alternative Names
Beta-A4 crystallin; beta-crystallin A4; crystallin, beta A4; crystallin, beta polypeptide A4; eye lens structural protein; MCOPCT4 CRYBA4 CTRCT23, CYRBA4, MCOPCT4 crystallin beta A4 beta-crystallin A4|beta crystallin A4 chain transcript PS|beta crystallin alpha 4 chain|beta-A4 crystallin|crystallin, beta polypeptide A4|eye lens structural protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on CRYBA4, check out the CRYBA4 Infographic
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In this infographic, you will see the following information for CRYBA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CRYBA4 (NM_001886) Human Over-expression Lysate (LS001413)
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No publications found for LS001413
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