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Product Info Summary
| SKU: | LS010212 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 (DDX39) |
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Product info
Product Name
DDX39 (DDX39A) (NM_005804) Human Over-expression Lysate
SKU/Catalog Number
LS010212
Size
100 µg
Description
Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 (DDX39)
Storage & Handling
Cite This Product
DDX39 (DDX39A) (NM_005804) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010212)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DDX39 (DDX39A) Human Over-expression Lysates NM_005804.
Western blot validation of overexpression lysate (LS010212) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DDX39A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DDX39A (Source: Uniprot.org, NCBI)
Gene Name
DDX39A
Full Name
ATP-dependent RNA helicase DDX39A
Weight
49.13kDa
Superfamily
DEAD box helicase family
Alternative Names
ATP-dependent RNA helicase DDX39; BAT1; BAT1L; DDX39; DDXL; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 transcript; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39; DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A; DEAD box protein 39; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39; EC 3.6.4.13; MGC18203; MGC8417; Nuclear RNA helicase URH49; nuclear RNA helicase, DECD variant of DEAD box family; UAP56-related helicase, 49 kDa; URH49 DDX39A BAT1, BAT1L, DDX39, DDXL, URH49 DExD-box helicase 39A ATP-dependent RNA helicase DDX39A|DEAD (Asp-Glu-Ala-Asp) box polypeptide 39A|DEAD box protein 39|DEAD-box helicase 39A|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 39|UAP56-related helicase, 49 kDa|nuclear RNA helicase URH49|nuclear RNA helicase, DECD variant of DEAD box family
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DDX39A, check out the DDX39A Infographic
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In this infographic, you will see the following information for DDX39A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DDX39 (DDX39A) (NM_005804) Human Over-expression Lysate (LS010212)
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No publications found for LS010212
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