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Product Info Summary
| SKU: | LS011325 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 |
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Product info
Product Name
DDX42 (NM_203499) Human Over-expression Lysate
SKU/Catalog Number
LS011325
Size
100 µg
Description
Transient overexpression lysate of DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2
Storage & Handling
Cite This Product
DDX42 (NM_203499) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011325)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DDX42 Human Over-expression Lysates NM_203499.
Western blot validation of overexpression lysate (LS011325) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DDX42 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DDX42 (Source: Uniprot.org, NCBI)
Gene Name
DDX42
Full Name
ATP-dependent RNA helicase DDX42
Weight
102.975kDa
Superfamily
DEAD box helicase family
Alternative Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box protein 42; EC 3.6.1; EC 3.6.4.13; RHELPSF3b DEAD box protein; RNA helicase-like protein; RNA helicase-related protein; RNAHPFLJ43179; SF3b125 DEAD-box protein; SF3b125ATP-dependent RNA helicase DDX42; Splicing factor 3B-associated 125 kDa protein DDX42 DDX42P, RHELP, RNAHP, SF3B8, SF3b125 DEAD-box helicase 42 ATP-dependent RNA helicase DDX42|DEAD (Asp-Glu-Ala-Asp) box helicase 42|DEAD (Asp-Glu-Ala-Asp) box polypeptide 42|SF3b DEAD box protein|splicing factor 3B-associated 125 kDa protein|splicing factor 3b, subunit 8
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DDX42, check out the DDX42 Infographic
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In this infographic, you will see the following information for DDX42: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DDX42 (NM_203499) Human Over-expression Lysate (LS011325)
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No publications found for LS011325
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