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Product Info Summary
| SKU: | LS011067 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of chromosome 10 open reading frame 10 (C10orf10) |
Product info
Product Name
DEPP1 (NM_007021) Human Over-expression Lysate
SKU/Catalog Number
LS011067
Size
100 µg
Description
Transient overexpression lysate of chromosome 10 open reading frame 10 (C10orf10)
Storage & Handling
Cite This Product
DEPP1 (NM_007021) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011067)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DEPP1 Human Over-expression Lysates NM_007021.
Western blot validation of overexpression lysate (LS011067) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with C10orf10 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DEPP1 (Source: Uniprot.org, NCBI)
Gene Name
DEPP1
Full Name
Protein DEPP1
Weight
23.406kDa
Alternative Names
Protein DEPP1 DEPP1 C10orf10, DEPP, FIG, Fseg DEPP1 autophagy regulator protein DEPP1|decidual protein induced by progesterone|fasting induced|fasting-induced gene protein|fasting-induced protein|fat-specific expressed|protein DEPP
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DEPP1, check out the DEPP1 Infographic
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In this infographic, you will see the following information for DEPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For DEPP1 (NM_007021) Human Over-expression Lysate (LS011067)
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No publications found for LS011067
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