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Product Info Summary
| SKU: | LS055184 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of chromosome 20 open reading frame 12 (C20orf12) |
Product info
Product Name
DZANK1 (NM_001099407) Human Over-expression Lysate
SKU/Catalog Number
LS055184
Size
100 µg
Description
Transient overexpression lysate of chromosome 20 open reading frame 12 (C20orf12)
Storage & Handling
Cite This Product
DZANK1 (NM_001099407) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS055184)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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DZANK1 Human Over-expression Lysates NM_001099407.
Western blot validation of overexpression lysate (LS055184) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DZANK1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for DZANK1 (Source: Uniprot.org, NCBI)
Gene Name
DZANK1
Full Name
Double zinc ribbon and ankyrin repeat-containing protein 1
Weight
82.192kDa
Alternative Names
ANKRD64; bA189K21.1; bA189K21.8; C20orf12; C20orf84; dJ568F9.2; double zinc ribbon and ankyrin repeat domains 1 DZANK1 ANKRD64, C20orf12, C20orf84 double zinc ribbon and ankyrin repeat domains 1 double zinc ribbon and ankyrin repeat-containing protein 1|ankyrin repeat domain 64|ankyrin repeat-containing protein C20orf12
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on DZANK1, check out the DZANK1 Infographic
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Specific Publications For DZANK1 (NM_001099407) Human Over-expression Lysate (LS055184)
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No publications found for LS055184
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