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Product Info Summary
| SKU: | LS010436 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of EMG1 nucleolar protein homolog (S. cerevisiae) (EMG1) |
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Product info
Product Name
EMG1 (NM_006331) Human Over-expression Lysate
SKU/Catalog Number
LS010436
Size
100 µg
Description
Transient overexpression lysate of EMG1 nucleolar protein homolog (S. cerevisiae) (EMG1)
Storage & Handling
Cite This Product
EMG1 (NM_006331) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS010436)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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EMG1 Human Over-expression Lysates NM_006331.
Western blot validation of overexpression lysate (LS010436) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with EMG1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for EMG1 (Source: Uniprot.org, NCBI)
Gene Name
EMG1
Full Name
Ribosomal RNA small subunit methyltransferase NEP1
Weight
26.72kDa
Superfamily
class IV-like SAM-binding methyltransferase superfamily
Alternative Names
18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; BWCNS; C2F18S rRNA Psi1248 methyltransferase; EMG1 nucleolar protein homolog (S. cerevisiae); essential for mitotic growth 1; FLJ60792; Grcc2f; NEP1EC 2.1.1.-; Nucleolar protein EMG1 homolog; probable ribosome biogenesis protein NEP1; Protein C2f; Ribosome biogenesis protein NEP1 EMG1 C2F, Grcc2f, NEP1 EMG1 N1-specific pseudouridine methyltransferase ribosomal RNA small subunit methyltransferase NEP1|18S rRNA (pseudouridine(1248)-N1)-methyltransferase|18S rRNA (pseudouridine-N1-)-methyltransferase NEP1|18S rRNA Psi1248 methyltransferase|EMG1 nucleolar protein homolog|essential for mitotic growth 1|nucleolar essential protein 1|ribosome biogenesis protein NEP1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on EMG1, check out the EMG1 Infographic
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In this infographic, you will see the following information for EMG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For EMG1 (NM_006331) Human Over-expression Lysate (LS010436)
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No publications found for LS010436
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