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Product Info Summary
| SKU: | LS008220 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of DiGeorge syndrome critical region gene 14 (DGCR14) |
Product info
Product Name
ESS2 (NM_022719) Human Over-expression Lysate
SKU/Catalog Number
LS008220
Size
100 µg
Description
Transient overexpression lysate of DiGeorge syndrome critical region gene 14 (DGCR14)
Storage & Handling
Cite This Product
ESS2 (NM_022719) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS008220)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ESS2 Human Over-expression Lysates NM_022719.
Western blot validation of overexpression lysate (LS008220) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with DGCR14 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ESS2 (Source: Uniprot.org, NCBI)
Gene Name
ESS2
Full Name
Splicing factor ESS-2 homolog
Weight
52.568kDa
Superfamily
ESS2 family
Alternative Names
Splicing factor ESS-2 homolog ESS2 DGCR13, DGCR14, DGS-H, DGS-I, DGSH, DGSI, ES2, ESS-2, Es2el, bis1 ess-2 splicing factor homolog splicing factor ESS-2 homolog|DiGeorge syndrome critical region gene 13|DiGeorge syndrome critical region gene 14|DiGeorge syndrome critical region gene DGSI|DiGeorge syndrome gene H|DiGeorge syndrome gene I|Protein DGCR13|diGeorge syndrome protein H|protein DGCR14
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ESS2, check out the ESS2 Infographic
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Specific Publications For ESS2 (NM_022719) Human Over-expression Lysate (LS008220)
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