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Product Info Summary
| SKU: | LS084985 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of family with sequence similarity 83, member A (FAM83A), transcript variant 1 |
Product info
Product Name
FAM83A (NM_032899) Human Over-expression Lysate
SKU/Catalog Number
LS084985
Size
100 µg
Description
Transient overexpression lysate of family with sequence similarity 83, member A (FAM83A), transcript variant 1
Storage & Handling
Cite This Product
FAM83A (NM_032899) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084985)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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FAM83A Human Over-expression Lysates NM_032899.
Western blot validation of overexpression lysate (LS084985) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM83A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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FAM83A Human Over-expression Lysates NM_207006.
Western blot validation of overexpression lysate (LS084985) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with FAM83A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for FAM83A (Source: Uniprot.org, NCBI)
Gene Name
FAM83A
Full Name
Protein FAM83A
Weight
47.458kDa
Superfamily
FAM83 family
Alternative Names
Protein FAM83A FAM83A BJ-TSA-9 family with sequence similarity 83 member A protein FAM83A|tumor antigen BJ-TSA-9|tumor-specific gene expressed in prostate protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on FAM83A, check out the FAM83A Infographic
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In this infographic, you will see the following information for FAM83A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For FAM83A (NM_032899) Human Over-expression Lysate (LS084985)
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No publications found for LS084985
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